Evidence map›Paper›PMID 35440683›Full record

ArticleCommunications biology2022

Silencing of STE20-type kinase STK25 in human aortic endothelial and smooth muscle cells is atheroprotective.

Emmelie Cansby, Sima Kumari, Mara Caputo, Ying Xia, Rando Porosk, Jonathan Robinson, Hao Wang, Britt-Marie Olsson, Josefine Vallin, Julie Grantham and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 5 institutions in 2 countries.

Emmelie CansbyDepartment of Chemistry and Molecular Biology, University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden.ORCID 0000-0003-1589-3596
Sima KumariDepartment of Chemistry and Molecular Biology, University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden.
Mara CaputoDepartment of Chemistry and Molecular Biology, University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden.
Ying XiaDepartment of Chemistry and Molecular Biology, University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden.
Rando PoroskDepartment of Biochemistry, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
Jonathan RobinsonDepartment of Biology and Biological Engineering, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Chalmers University of Technology, Gothenburg, Sweden.
Hao WangDepartment of Biology and Biological Engineering, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Chalmers University of Technology, Gothenburg, Sweden.ORCID 0000-0001-7475-0136
Britt-Marie OlssonProteomics Core Facility, University of Gothenburg, Gothenburg, Sweden.
Josefine VallinDepartment of Chemistry and Molecular Biology, University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden.
Julie GranthamDepartment of Chemistry and Molecular Biology, University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden.
Ursel SoometsDepartment of Biochemistry, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.ORCID 0000-0002-3681-188X
L Thomas SvenssonDepartment of Biology and Biological Engineering, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Chalmers University of Technology, Gothenburg, Sweden.
Carina SihlbomProteomics Core Facility, University of Gothenburg, Gothenburg, Sweden.
Hanns-Ulrich MarschallDepartment of Molecular and Clinical Medicine/Wallenberg Laboratory, Institute of Medicine, University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden.ORCID 0000-0001-7347-3085
Andreas EdsfeldtDepartment of Clinical Sciences Malmö, Clinical Research Center, Lund University, Malmö, Sweden.
Isabel GoncalvesDepartment of Clinical Sciences Malmö, Clinical Research Center, Lund University, Malmö, Sweden.
Margit MahlapuuDepartment of Chemistry and Molecular Biology, University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden. Margit.Mahlapuu@gu.se.ORCID 0000-0001-8740-8874
Sahlgrenska University Hospital · SEScience for Life Laboratory · SELund University · SEUniversity of Gothenburg · SEUniversity of Tartu · EE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent studies highlight the importance of lipotoxic damage in aortic cells as the major pathogenetic contributor to atherosclerotic disease. Since the STE20-type kinase STK25 has been shown to exacerbate ectopic lipid storage and associated cell injury in several metabolic organs, we here investigate its role in the main cell types of vasculature. We depleted STK25 by small interfering RNA in human aortic endothelial and smooth muscle cells exposed to oleic acid and oxidized LDL. In both cell types, the silencing of STK25 reduces lipid accumulation and suppresses activation of inflammatory and fibrotic pathways as well as lowering oxidative and endoplasmic reticulum stress. Notably, in smooth muscle cells, STK25 inactivation hinders the shift from a contractile to a synthetic phenotype. Together, we provide several lines of evidence that antagonizing STK25 signaling in human aortic endothelial and smooth muscle cells is atheroprotective, highlighting this kinase as a new potential therapeutic target for atherosclerotic disease.

Indexed as

AtherosclerosisIntracellular Signaling Peptides and ProteinsHippo KinasesHumansLipid MetabolismLipidsMyocytes, Smooth MuscleProtein Serine-Threonine KinasesHippo KinasesIntracellular Signaling Peptides and ProteinsLipidsProtein Serine-Threonine KinasesSTK25 protein, human

Identifiers

PMID35440683
PMCPMC9018782
OpenAlexW4224248844

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.