ArticleNPJ biofilms and microbiomes2022
Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics.
Article in NPJ biofilms and microbiomes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Gut OncoMicrobiome Signatures (GOMS) as next-generation biomarkers for cancer immunotherapy.Nature reviews. Clinical oncology · 2023Pooled it
- The role of gut microbiome in aging-associated diseases: where do we stand now and how technology will transform the future.Gut microbes · 2026Review
- Bridging the gap:Gut microbes · 2026Review
- The gut microbiome and ageing trajectories: mechanisms and clinical implications.Nature reviews. Endocrinology · 2026Review
- Processed Diets and Food Additives Shape the Gut Microbiota and Chronic Disease Risk Across the Life Course-A Three-Layer Ecosystem Disruption Model (TLED) Model.Life (Basel, Switzerland) · 2026Review
- Hainan Longevity Model, Senile Degeneration, Cognitive Disability and Healthy Longevity.Aging medicine (Milton (N.S.W)) · 2026Review
- First metagenomic analysis of age-associated changes in the gut microbiome among healthy Saudi adults: SAMS pilot study.Frontiers in aging · 2026Article
- Synergy between culturomics and metagenomics of health status-associated gut bacteria originating from non-IBD and IBD populations.Scientific reports · 2025Article
- Expanded gut microbial genomes from Chinese populations reveal population-specific genomic features related to human physiological traits.Genome medicine · 2025Article
- Human gut Bifidobacteria strains regulate host longevity via shared and divergent mechanisms inbioRxiv : the preprint server for biology · 2025Article
- Severe Cognitive Decline in Long-term Care Is Related to Gut Microbiome Production of Metabolites Involved in Neurotransmission, Immunomodulation, and Autophagy.The journals of gerontology. Series A, Biological sciences and medical sciences · 2025Article
- Investigating the gut bacteria structure and function of hibernating bats through 16S rRNA high-throughput sequencing and culturomics.mSystems · 2025Article
- Age-related dynamics of predominant methanogenic archaea in the human gut microbiome.BMC microbiology · 2025Article
- Phenotypic upregulation of hexocylceramides and ether-linked phosphocholines as markers of human extreme longevity.Aging cell · 2025Article
- Reevaluation of the gastrointestinal methanogenic archaeome in multiple sclerosis and its association with treatment.Microbiology spectrum · 2025Article
- Gut microbiota and healthy longevity.Science China. Life sciences · 2024Review
- Advances in the isolation, cultivation, and identification of gut microbes.Military Medical Research · 2024Review
- Divergent age-associated and metabolism-associated gut microbiome signatures modulate cardiovascular disease risk.Nature medicine · 2024Article
- Gut microbiota in centenarians: A potential metabolic and aging regulator in the study of extreme longevity.Aging medicine (Milton (N.S.W)) · 2024Review
- Aging Gut Microbiome in Healthy and Unhealthy Aging.Aging and disease · 2024Review
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gut microbes play important roles in human longevity and the gut microbiota profile of centenarians shows some unique features from young adults. Nowadays, most microbial studies on longevity are commonly based on metagenomic sequencing which may lose information about the functional microbes with extremely low abundance. Here, we combined in-depth metagenomic sequencing and large-scale culturomics to reveal the unique gut microbial structure of a Chinese longevity population, and to explore the possible relationship between intestinal microbes and longevity. Twenty-five healthy Hainan natives were enrolled in the study, including 12 centenarians and 13 senior neighbors. An average of 51.1 Gb raw sequencing data were obtained from individual fecal sample. We assembled 1778 non-redundant metagenomic assembled genomes (MAGs), 33.46% of which cannot be classified into known species. Comparison with the ordinary people in Hainan province, the longevous cohort displayed significantly decreased abundance of butyrate-producing bacteria and largely increased proportion of Escherichia coli, Desulfovibrio piger and Methanobrevibacter smithii. These species showed a constant change with aging. We also isolated 8,030 strains from these samples by large-scale culturomics, most of which belonged to 203 known species as identified by MALDI-TOF. Surprisingly, only 42.17% of the isolated species were also detected by metagenomics, indicating obvious complementarity between these two approaches. Combination of two complement methods, in-depth metagenomic sequencing and culturomics, provides deeper insights into the longevity-related gut microbiota. The uniquely enriched gut microbes in Hainan extreme decades population may help to promote health and longevity.
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