ArticleMicrobial genomics2022
High molecular weight DNA extraction methods lead to high quality filamentous ascomycete fungal genome assemblies using Oxford Nanopore sequencing.
Article in Microbial genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 29 citations in OpenAlex.
- Isolation, Biocontrol Efficacy and Genomic Analysis ofMicroorganisms · 2026Article
- Quantitative comparison of fungal genome assembly strategies using short and long reads from simulated and empirical sequencing data.Microbial genomics · 2026Article
- Nocardia fodinahabitans sp. nov. isolated from underground hard coal mine waters shows biotechnological potential for degradation of aromatic hydrocarbons.Molecular genetics and genomics : MGG · 2026Article
- Genome sequence ofMicrobiology resource announcements · 2026Article
- An automated decision-making procedure for ranking and selecting species in biodiversity projects.npj biodiversity · 2026Article
- Impact of DNA Extraction Strategies on Genomic and Bioinformatic Outcomes in Eight Selected Fungal Strains.Journal of fungi (Basel, Switzerland) · 2026Article
- Analysis of 21 Apiospora genomes reveals a genus with tremendous synthesis potential for carbohydrate-active enzymes and secondary metabolites.BMC genomics · 2026Article
- 2A peptides enable simplified discovery and heterologous production of fungal bioactive metabolites in Yarrowia lipolytica.Applied microbiology and biotechnology · 2026Article
- A deep dive into the diversity of theFrontiers in public health · 2026Article
- Benchmarking Cost-Effective DNA Extraction Kits for Diverse Metagenomic Samples.International journal of molecular sciences · 2025Article
- Gapless near Telomer-to-Telomer Assembly ofJournal of fungi (Basel, Switzerland) · 2025Article
- Insights into Persian Gulf Beach Sand Mycobiomes: Promises and Challenges in Fungal Diversity.Journal of fungi (Basel, Switzerland) · 2025Article
- Article
- Fusarium Genome Sequencing and Assembly.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Identification of a terpene synthase arsenal using long-read sequencing and genome assembly of Aspergillus wentii.BMC genomics · 2024Article
- Single Laboratory Evaluation of the Q20+ Nanopore Sequencing Kit for Bacterial Outbreak Investigations.International journal of molecular sciences · 2024Article
- Evaluation of Commercial RNA Extraction Protocols for Avian Influenza Virus Using Nanopore Metagenomic Sequencing.Viruses · 2024Article
- Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches.Journal of fungi (Basel, Switzerland) · 2024Review
- Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites.IMA fungus · 2024Article
- First whole-genome sequence and assembly of the Ecuadorian brown-headed spider monkey (Ateles fusciceps fusciceps), a critically endangered species, using Oxford Nanopore Technologies.G3 (Bethesda, Md.) · 2024Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the last two decades, whole-genome sequencing has revolutionized genetic research in all kingdoms, including fungi. More than 1000 fungal genomes have been submitted to sequence databases, mostly obtained through second generation short-read DNA sequencing. As a result, highly fragmented genome drafts have typically been obtained. However, with the emergence of third generation long-read DNA sequencing, the assembly challenge can be overcome and highly contiguous assemblies obtained. Such attractive results, however, are extremely dependent on the ability to extract highly purified high molecular weight (HMW) DNA. Extraction of such DNA is currently a significant challenge for all species with cell walls, not least fungi. In this study, four isolates of filamentous ascomycetes (
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