Evidence map›Paper›PMID 35438371›Full record

ArticleJournal of fluorescence2022

Fluorescent indicators for live-cell and in vitro detection of inorganic cadmium dynamics.

Shulin Hu, Jun Yang, Anqi Liao, Ying Lin, Shuli Liang

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shulin HuGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, 510006, Guangzhou, People's Republic of China.
Jun YangGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, 510006, Guangzhou, People's Republic of China.
Anqi LiaoGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, 510006, Guangzhou, People's Republic of China.
Ying LinGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, 510006, Guangzhou, People's Republic of China.
Shuli LiangGuangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, 510006, Guangzhou, People's Republic of China. shuli@scut.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cadmium contamination is a severe threat to the environment and food safety. Thus, there is an urgent need to develop highly sensitive and selective cadmium detection tools. The engineered fluorescent indicator is a powerful tool for the rapid detection of inorganic cadmium in the environment. In this study, the development of yellow fluorescent indicators of cadmium chloride by inserting a fluorescent protein at different positions of the high cadmium-specific repressor and optimizing the flexible linker between the connection points is reported. These indicators provide a fast, sensitive, specific, high dynamic range, and real-time readout of cadmium ion dynamics in solution. The excitation and emission wavelength of this indicator used in this work are 420/485 and 528 nm, respectively. Fluorescent indicators N0C0/N1C1 showed a linear response to cadmium concentration within the range from 10/30 to 50/100 nM and with a detection limit of 10/33 nM under optimal condition. Escherichia coli cells containing the indicator were used to further study the response of cadmium ion concentration in living cells. E. coli N1C1 could respond to different concentrations of cadmium ions. This study provides a rapid and straightforward method for cadmium ion detection in vitro and the potential for biological imaging.

Indexed as

CadmiumFluorescent DyesEscherichia coliIonsProteinsCadmiumFluorescent DyesIonsProteinsDynamicsFluorescent indicatorInorganic cadmiumIn vitroLive-cell

Identifiers

PMID35438371

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.