ArticleThe Plant journal : for cell and molecular biology2022
Insights into acylation mechanisms: co-expression of serine carboxypeptidase-like acyltransferases and their non-catalytic companion paralogs.
Article in The Plant journal : for cell and molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 70 citations in OpenAlex.
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- Integrative Multi-Omics Elucidates the Molecular Mechanisms Underlying Sweet-Mellow Taste Formation During Withering of Niangniang Tea (Large-Leaf Yellow Tea).Foods (Basel, Switzerland) · 2026Article
- Article
- Completing the acylation landscape in plants: GDSL enzymes join the BAHD and SCPL acyltransferase families.Journal of experimental botany · 2026Review
- Integrated metabolomic and transcriptomic analysis of Camellia sinensis var. pubilimba 'Rucheng Baimaocha' reveals distinct flavonoid and strictinin biosynthesis.BMC plant biology · 2026Article
- Identification of a unique tea germplasm exhibiting hyperaccumulation of trans-catechins and its molecular regulatory mechanism.Food chemistry. Molecular sciences · 2026Article
- Morphological and Chemical Characterization of a Novel Wild Tea Plant Resource with Naturally Low Caffeine and High Theobromine from Guangxi Province, China.Plants (Basel, Switzerland) · 2026Article
- Mass Spectrometry Imaging-Assisted Discovery of Gallotannin Biosynthetic Genes in the Root of Paeonia suffruticosa.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Unravelling the genetics of heat-responsive fruit traits in pepper [Capsicum annuum L.] via GWAS under subtropical climates.BMC plant biology · 2026Article
- Metabolome and transcriptome analyses reveal metabolic differences and identify candidate regulatory factors among three habitat types ofFrontiers in plant science · 2026Article
- Biosynthesis and Application of Catechins and Their Derivatives inFood science & nutrition · 2025Review
- Functional divergence of tandem duplicate SCPL acyltransferase genes reveals convergent evolution of chicoric acid biosynthesis.Science advances · 2025Article
- Elevated atmospheric CO2-induced reprogramming leads to decreased seed protein and nutritional quality in forest trees.Plant physiology · 2025Article
- Influencing Factors and Regulatory Mechanisms of Fresh Tea Leaf Quality: A Review.Foods (Basel, Switzerland) · 2025Review
- Identification of QTLs controlling hydrolyzable tannin contents derived from a wild tea relative,Breeding science · 2025Article
- A Comprehensive Review of Phenolic Compounds in Horticultural Plants.International journal of molecular sciences · 2025Review
- Two shikimate dehydrogenases play an essential role in the biosynthesis of galloylated catechins in tea plants.Horticulture research · 2025Article
- Key genes in a "Galloylation-Degalloylation cycle" controlling the synthesis of hydrolyzable tannins in strawberry plants.Horticulture research · 2025Article
- Deactivating mutations in the catalytic site of a companion serine carboxypeptidase-like acyltransferase enhance catechin galloylation inHorticulture research · 2025Article
- Identifying meteorological factors influencing catechin biosynthesis and optimizing cultivation conditions of tea plant (Frontiers in plant science · 2025Article
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Authors and funding
16 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serine carboxypeptidase-like acyltransferases (SCPL-ATs) play a vital role in the diversification of plant metabolites. Galloylated flavan-3-ols highly accumulate in tea (Camellia sinensis), grape (Vitis vinifera), and persimmon (Diospyros kaki). To date, the biosynthetic mechanism of these compounds remains unknown. Herein, we report that two SCPL-AT paralogs are involved in galloylation of flavan-3-ols: CsSCPL4, which contains the conserved catalytic triad S-D-H, and CsSCPL5, which has the alternative triad T-D-Y. Integrated data from transgenic plants, recombinant enzymes, and gene mutations showed that CsSCPL4 is a catalytic acyltransferase, while CsSCPL5 is a non-catalytic companion paralog (NCCP). Co-expression of CsSCPL4 and CsSCPL5 is likely responsible for the galloylation. Furthermore, pull-down and co-immunoprecipitation assays showed that CsSCPL4 and CsSCPL5 interact, increasing protein stability and promoting post-translational processing. Moreover, phylogenetic analyses revealed that their homologs co-exist in galloylated flavan-3-ol- or hydrolyzable tannin-rich plant species. Enzymatic assays further revealed the necessity of co-expression of those homologs for acyltransferase activity. Evolution analysis revealed that the mutations of the CsSCPL5 catalytic residues may have taken place about 10 million years ago. These findings show that the co-expression of SCPL-ATs and their NCCPs contributes to the acylation of flavan-3-ols in the plant kingdom.
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