ArticlePlant methods2022
Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants.
Article in Plant methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 26 citations in OpenAlex.
- Screening of Rhizobacteria Capable of Modulating Cd Accumulation and Enhancing Pharmacopoeial Constituents inPlants (Basel, Switzerland) · 2026Article
- Functional characterization of SmNRAMP1 allelic variants conferring reduced cadmium uptake in Salvia miltiorrhiza.BMC plant biology · 2026Article
- Ecotype-specific phenolic acid accumulation and root softness in Salvia miltiorrhiza are driven by environmental and genetic factors.Plant biotechnology journal · 2025Article
- Multisource spectral fusion combined with variable selection for rapid geographical origin discrimination ofFrontiers in chemistry · 2025Article
- Characterization of the Molecular Events Underlying the Establishment of Axillary Meristem Region in Pepper.International journal of molecular sciences · 2023Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2023Article
- Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility inInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
Abstract
bcakgroundThe dry root and rhizome of Salvia miltiorrhiza are used to treat cardiovascular diseases, chronic pain, and thoracic obstruction over 2000 years in Asian countries. For high quality, Sichuan Zhongjiang is regarded as the genuine producing area of S. miltiorrhiza. Given its abnormal pollen development, S. miltiorrhiza from Sichuan (S.m.-SC) relies on root reproduction and zymad accumulation; part of diseased plants present typical viral disease symptoms and seed quality degeneration. This study aim to detected unknown viruses from mosaic-diseased plants and establish a highly efficient virus-free regeneration system to recover germplasm properties.
resultsTobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) were detected from mosaic-diseased plants. Primary apical meristem with two phyllo podium in 0.15-0.5 mm peeled from diseased plants were achieved 73.33% virus-free rate. The results showed that the medium containing MS, 0.5 mg/L 6-BA, 0.1 mg/L NAA, 0.1 mg/L GA
conclusionsA highly efficient virus-free regeneration system of S.m.-SC was established based on the detected viruses to recover superior seed quality. The proposed system laid support to control disease spread, recover good germplasm properties in S.m.-SC.
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