ArticleMicrobial biotechnology2022
Identification of genome integration sites for developing a CRISPR-based gene expression toolkit in Yarrowia lipolytica.
Article in Microbial biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Structure-coevolution dual-guided engineering of C-glycosyltransferase enables high-level biosynthesis of vitexin inSynthetic and systems biotechnology · 2026Article
- EngineeringSynthetic and systems biotechnology · 2026Article
- Efficient production of astaxanthin inSynthetic and systems biotechnology · 2025Article
- Unlocking the Synthetic Potential of Yarrowia lipolytica: Innovating Gene Expression Tools.Microbial biotechnology · 2025Review
- Construction of an Aspergillus oryzae △nptB△pyrG Host for Homologous Expression of Lipase and Catalytic Property Characterization of Recombinant Lipase.Applied biochemistry and biotechnology · 2025Article
- Advances and perspectives in genetic expression and operation for the oleaginous yeastSynthetic and systems biotechnology · 2024Review
- Optimizing multicopy chromosomal integration for stable high-performing strains.Nature chemical biology · 2024Article
- De Novo Synthesis of Resveratrol from Sucrose by Metabolically EngineeredBiomolecules · 2024Article
- Strategies for the efficient biosynthesis of β-carotene through microbial fermentation.World journal of microbiology & biotechnology · 2024Review
- Genome-scale transcriptional activation by non-homologous end joining-mediated integration in Yarrowia lipolytica.Biotechnology for biofuels and bioproducts · 2024Article
- Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica.Nature communications · 2023Article
- Genetic tools for metabolic engineering ofEngineering microbiology · 2023Review
- Characterization of an efficient CRISPR-iCas9 system in Yarrowia lipolytica for the biosynthesis of carotenoids.Applied microbiology and biotechnology · 2023Article
- Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts.Synthetic and systems biotechnology · 2023Review
- Genome-wide analysis reveals Hsf1 maintains high transcript abundance of target genes controlled by strong constitutive promoter in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- A review of synthetic biology tools in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2023Review
- Identification of neutral genome integration sites with high expression and high integration efficiency inSynthetic and systems biotechnology · 2023Article
- Identification of genome integration sites for developing a CRISPR-based gene expression toolkit in Yarrowia lipolytica.Microbial biotechnology · 2022Article
- Efficient production of the β-ionone aroma compound from organic waste hydrolysates using an engineeredFrontiers in microbiology · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the rapid development of synthetic biology, the oleaginous yeast Yarrowia lipolytica has become an attractive microorganism for chemical production. To better optimize and reroute metabolic pathways, we have expanded the CRISPR-based gene expression toolkit of Y. lipolytica. By sorting the integration sites associated with high expression, new neutral integration sites associated with high expression and high integration efficiency were identified. Diverse genetic components, including promoters and terminators, were also characterized to expand the expression range. We found that in addition to promoters, the newly characterized terminators exhibited large variations in gene expression. These genetic components and integration sites were then used to regulate genes involved in the lycopene biosynthesis pathway, and different levels of lycopene production were achieved. The CRISPR-based gene expression toolkit developed in this study will facilitate the genetic engineering of Y. lipolytica.
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