ArticleApplied and environmental microbiology2022
Microwell Fluoride Screen for Chemical, Enzymatic, and Cellular Reactions Reveals Latent Microbial Defluorination Capacity for -CF
Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- A hydrolase from Serratia liquefaciens IMD717 with esterase, dehalogenase and N-deformylase activities.Applied microbiology and biotechnology · 2026Article
- Engineering Fluoroacetate Dehalogenase by Growth-Based Selections on Non-Natural Organofluorides.Angewandte Chemie (International ed. in English) · 2026Article
- Article
- Continuous Spectrophotometric Assay for Defluorinase and Dechlorinase Activities With α-Halocarboxylic Acids.Microbial biotechnology · 2025Article
- Enzymatic carbon-fluorine bond cleavage by human gut microbes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lanmodulin-Decorated Microbes for Efficient Lanthanide Recovery.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- A prescription for engineering PFAS biodegradation.The Biochemical journal · 2024Review
- Defluorination of monofluorinated alkane by Rhodococcus sp. NJF-7 isolated from soil.AMB Express · 2024Article
- Evolutionary obstacles and not C-F bond strength make PFAS persistent.Microbial biotechnology · 2024Article
- Biodegradation of Amphipathic Fluorinated Peptides Reveals a New Bacterial Defluorinating Activity and a New Source of Natural Organofluorine Compounds.Environmental science & technology · 2023Article
- Fluoro-recognition: New in vivo fluorescent assay for toluene dioxygenase probing induction by and metabolism of polyfluorinated compounds.Environmental microbiology · 2022Article
- Strategies for the Biodegradation of Polyfluorinated Compounds.Microorganisms · 2022Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The capacity to defluorinate polyfluorinated organic compounds is a rare phenotype in microbes but is increasingly considered important for maintaining the environment. New discoveries will be greatly facilitated by the ability to screen many natural and engineered microbes in a combinatorial manner against large numbers of fluorinated compounds simultaneously. Here, we describe a low-volume, high-throughput screening method to determine defluorination capacity of microbes and their enzymes. The method is based on selective binding of fluoride to a lanthanum chelate complex that gives a purple-colored product. It was miniaturized to determine biodefluorination in 96-well microtiter plates by visual inspection or robotic handling and spectrophotometry. Chemicals commonly used in microbiological studies were examined to define usable buffers and reagents. Base-catalyzed, purified enzyme and whole-cell defluorination reactions were demonstrated with fluoroatrazine and showed correspondence between the microtiter assay and a fluoride electrode. For discovering new defluorination reactions and mechanisms, a chemical library of 63 fluorinated compounds was screened
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