Evidence map›Paper›PMID 35435456›Full record

ArticleApplied microbiology and biotechnology2022

Hypersecretion of OmlA antigen in Corynebacterium glutamicum through high-throughput based development process.

Manman Sun, Alex Xiong Gao, Rodrigo Ledesma-Amaro, An Li, Rongbin Wang, Jianqi Nie, Pei Zheng, Yankun Yang, Zhonghu Bai, Xiuxia Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Engineered membraneless organelles inSynthetic and systems biotechnology · 2025
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 4 countries.

Manman SunNational Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214112, China.
Alex Xiong GaoDivision of Life Science, The Hong Kong University of Science and Technology, Hong Kong, China.
Rodrigo Ledesma-AmaroDepartment of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, SW7 2AZ, UK.
An LiNational Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214112, China.
Rongbin WangDepartment of Life Technologies, University of Turku, 20014, Turku, Finland.
Jianqi NieNational Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214112, China.
Pei ZhengTecon Biology CO.Ltd, Urumqi, 830000, China.
Yankun YangNational Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214112, China.
Zhonghu BaiNational Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214112, China.
Xiuxia LiuNational Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214112, China. liuxiuxia@jiangnan.edu.cn.
Jiangnan University · CNHong Kong University of Science and Technology · HKImperial College London · GBUniversity of Turku · FI

Funding

BBSRC BB/T013176/1British Council 527429894National First-Class Discipline Program of Light Industry Technology and Engineering LITE2018-24National Natural Science Foundation of China 21878124National Natural Science Foundation of China 21938004National Natural Science Foundation of China No.22078128Postgraduate Research & Practice Innovation Program of Jiangsu Province KYCX21-2028the 111 Project No. 111-2-06the Fundamental Research Funds for the Central Universities No. JUSRP221032the Opening Project of the Key Laboratory of Industrial Biotechnology, Ministry of Education KLIBKF201802
6 · The paper itself

Abstract

Outer membrane lipoprotein A (OmlA) is a vaccine antigen against porcine contagious pleuropneumonia (PCP), a disease severely affecting the swine industry. Here, we aimed to systematically potentiate the secretory production of OmlA in Corynebacterium glutamicum (C. glutamicum), a widely used microorganism in the food industry, by establishing a holistic development process based on our high-throughput culture platform. The expression patterns, expression element combinations, medium composition, and induction conditions were comprehensively screened or optimized in microwell plates (MWPs), followed by fermentation parameter optimization in a 4 × 1 L parallel fermentation system (CUBER4). An unprecedented yield of 1.01 g/L OmlA was ultimately achieved in a 5-L bioreactor following the scaling-up strategy of fixed oxygen mass transfer coefficient (k

Indexed as

Actinobacillus pleuropneumoniaeCorynebacterium glutamicumAnimalsBioreactorsFermentationLipoprotein(a)SwineLipoprotein(a)BicistronCorynebacterium glutamicumHigh-throughput cultureOuter membrane lipoprotein AProcess development

Identifiers

PMID35435456
OpenAlexW4224126135

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.