ArticleApplied microbiology and biotechnology2022
Effects of mokF gene deletion and overexpression on the Monacolin K metabolism yields of Monascus purpureus.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 15 citations in OpenAlex.
- Nutritional Value Improvement of Oats by Solid-State Fermentation withFoods (Basel, Switzerland) · 2025Article
- The Role of Enoyl Reductase in the Monacolin K Biosynthesis Pathway inJournal of fungi (Basel, Switzerland) · 2025Article
- Combinatorial mutagenesis and fermentation optimization biotechnologies synergistically enhance monacolin K content in functional red yeast rice.Frontiers in microbiology · 2025Article
- Unlocking fungal quorum sensing: Oxylipins and yeast interactions enhance secondary metabolism inHeliyon · 2024Article
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Authors and funding
9 authors at 1 institution in 1 country.
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Abstract
Monascus purpureus is a fungus known for producing various physiologically active secondary metabolites. Of these, Monacolin K, a compound with hypocholesterolemic effects, is controlled by the biosynthetic gene mokF. Here, mokF deletion and overexpression strains (F2 and C3, respectively) were constructed using genetic engineering and compared with the M. purpureus wild strain (M1). The results showed that Monacolin K production was reduced by 50.86% in F2 and increased by 74.19% in C3. Of the three strains, C3 showed the highest production of Monacolin K and the most abnormal morphology. In addition, mokF influenced the expression level of mokA-mokI and might play an important role in regulating the biosynthesis of secondary metabolites in M. purpureus. Overall, our study verified the function of mokF in M. purpureus using gene deletion and overexpression technology. KEY POINTS: • The deletion and overexpression strains of mokF gene were successfully constructed. • The deletion or overexpression of mokF gene directly affected Monacolin K production. •The mokF gene had little effect on Monascus pigments and cell biomass.
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