ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022
Directing Multicellular Organization by Varying the Aspect Ratio of Soft Hydrogel Microwells.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- A 4D GelMA-Cad/Gelatin Hydrogel for High-Resolution DMD Bioprinting of Vascularized Soft Tissue Models.bioRxiv : the preprint server for biology · 2026Article
- Microengineered Gradient Hydrogels for Mechanobiology.Advanced healthcare materials · 2026Review
- Dynamic hydrogel mechanics in organoid engineering: From matrix design to translational paradigms.Bioactive materials · 2026Review
- Bioprinted Recombinant Human-Collagen-Based In Vitro Skin Models for Assessing Effects of Nano-ZnO on Dermis.Gels (Basel, Switzerland) · 2025Article
- Nano- and Microscale Chemical and Topographical Patterning of Synthetic Cell Scaffolds: from Hard to Soft Materials.ACS materials Au · 2025Review
- Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review.Molecular medicine (Cambridge, Mass.) · 2025Review
- Spatially defined microenvironment for engineering organoids.Biophysics reviews · 2024Review
- Facile Physicochemical Reprogramming of PEG-Dithiolane Microgels.Advanced healthcare materials · 2024Article
- Acute downregulation of emerin alters actomyosin cytoskeleton connectivity and function.Biophysical journal · 2023Article
- Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review.Biomaterials research · 2023Review
- Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Engineered hydrogels for mechanobiology.Nature reviews. Methods primers · 2022Article
- Directing Multicellular Organization by Varying the Aspect Ratio of Soft Hydrogel Microwells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Multicellular organization with precise spatial definition is essential to various biological processes, including morphogenesis, development, and healing in vascular and other tissues. Gradients and patterns of chemoattractants are well-described guides of multicellular organization, but the influences of 3D geometry of soft hydrogels are less well defined. Here, the discovery of a new mode of endothelial cell self-organization guided by combinatorial effects of stiffness and geometry, independent of protein or chemical patterning, is described. Endothelial cells in 2 kPa microwells are found to be ≈30 times more likely to migrate to the edge to organize in ring-like patterns than in stiff 35 kPa microwells. This organization is independent of curvature and significantly more pronounced in 2 kPa microwells with aspect ratio (perimeter/depth) < 25. Physical factors of cells and substrates that drive this behavior are systematically investigated and a mathematical model that explains the organization by balancing the dynamic interaction between tangential cytoskeletal tension, cell-cell, and cell-substrate adhesion is presented. These findings demonstrate the importance of combinatorial effects of geometry and stiffness in complex cellular organization that can be leveraged to facilitate the engineering of bionics and integrated model organoid systems with customized nutrient vascular networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.