Evidence map›Paper›PMID 35434655›Full record

ArticleSTAR protocols2022

An optimized protocol for phenotyping human granulocytes by mass cytometry.

Nora Vivanco Gonzalez, John-Paul Oliveria, Dmitry Tebaykin, Geoffrey T Ivison, Kaori Mukai, Mindy M Tsai, Luciene Borges, Kari C Nadeau, Stephen J Galli, Albert G Tsai and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Mass Cytometry in Food Allergy Research.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 2 countries.

Nora Vivanco GonzalezDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
John-Paul OliveriaDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Dmitry TebaykinDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Geoffrey T IvisonDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Kaori MukaiDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Mindy M TsaiDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Luciene BorgesDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Kari C NadeauSean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States.
Stephen J GalliDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Albert G TsaiDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Sean C BendallDepartment of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.
Stanford University · US

Funding

Stanford Cancer Immune Monitoring and Analysis Center (CIMAC)U24CA224309 · NCI · STANFORD UNIVERSITY · PI Sean Curtis Bendall, Holden T. Maecker · 2017 to 2026
$19.9M
Tracking allergen specific T cells in multi-food allergyU19AI104209 · NIAID · STANFORD UNIVERSITY · PI CHINTHRAJAH, R. SHARON · 2013 to 2025
$17.3M
Stanford/UNC Biomimetic U19 Research CenterU19AI116484 · NIAID · STANFORD UNIVERSITY · PI AMIEVA, MANUEL R, KUO, CALVIN J · 2015 to 2025
$13.5M
MIRIAD - Multiplexed Imaging of Resilience In Alzheimers DiseaseR01AG057915 · NIA · STANFORD UNIVERSITY · PI ANGELO, ROBERT MICHAEL, BENDALL, SEAN CURTIS · 2017 to 2021
$4.5M
Deciphering the Glycan Code in Human Alzheimer’s Disease BrainR01AG078702 · NIA · UNIVERSITY OF KENTUCKY · PI Peggi M Angel, Sean Curtis Bendall · 2022 to 2026
$3.8M
The Phenotypic Landscape of Cognitive Decline as Revealed by Next-Generation Multiplexed Ion Beam ImagingR01AG056287 · NIA · STANFORD UNIVERSITY · PI ANGELO, ROBERT MICHAEL, BENDALL, SEAN CURTIS · 2017 to 2021
$2.6M
A single-cell platform to discover and study regulators of human developmentR00GM104148 · NIGMS · STANFORD UNIVERSITY · PI BENDALL, SEAN CURTIS · 2014 to 2016
$747k
CIHRNCI NIH HHS U24 CA224309NIAID NIH HHS U19 AI104209NIAID NIH HHS U19 AI116484NIA NIH HHS R01 AG056287NIA NIH HHS R01 AG057915NIA NIH HHS R01 AG078702NIGMS NIH HHS R00 GM104148
6 · The paper itself

Abstract

Granulocytes encompass diverse roles, from fighting off pathogens to regulating inflammatory processes in allergies. These roles are represented by distinct cellular phenotypes that we captured with mass cytometry (CyTOF). Our protocol enables simultaneous evaluation of human basophils, eosinophils, and neutrophils under homeostasis and upon immune activation by anti-Immunoglobulin E (anti-IgE) or interleukin-3 (IL-3). Granulocyte integrity and detection of protein markers were optimized so that rare granulocyte populations could be deeply characterized by single cell mass cytometry. For complete details on the use and execution of this protocol, please refer to Vivanco Gonzalez et al. (2020).

Indexed as

EosinophilsNeutrophilsBasophilsFlow CytometryHumansLeukocyte CountAntibodyCell isolationFlow Cytometry/Mass CytometryHealth SciencesImmunologySingle Cell

Identifiers

PMID35434655
PMCPMC9010787
OpenAlexW4224244816

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.