Evidence map›Paper›PMID 35434559›Full record

ArticleiScience2022

The non-adrenergic imidazoline-1 receptor protein nischarin is a key regulator of astrocyte glutamate uptake.

Swati Gupta, Narges Bazargani, James Drew, Jack H Howden, Souvik Modi, Sana Al Awabdh, Hélène Marie, David Attwell, Josef T Kittler

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Swati GuptaDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
Narges BazarganiDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
James DrewDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
Jack H HowdenDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
Souvik ModiDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
Sana Al AwabdhDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
Hélène MarieDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
David AttwellDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
Josef T KittlerDepartment of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, WC1E 6BT London, UK.
University College London · GB

Funding

Wellcome Trust
6 · The paper itself

Abstract

Astrocytic GLT-1 is the main glutamate transporter involved in glutamate buffering in the brain, pivotal for glutamate removal at excitatory synapses to terminate neurotransmission and for preventing excitotoxicity. We show here that the surface expression and function of GLT-1 can be rapidly modulated through the interaction of its N-terminus with the nonadrenergic imidazoline-1 receptor protein, Nischarin. The phox domain of Nischarin is critical for interaction and internalization of surface GLT-1. Using live super-resolution imaging, we found that glutamate accelerated Nischarin-GLT-1 internalization into endosomal structures. The surface GLT-1 level increased in Nischarin knockout astrocytes, and this correlated with a significant increase in transporter uptake current. In addition, Nischarin knockout in astrocytes is neuroprotective against glutamate excitotoxicity. These data provide new molecular insights into regulation of GLT-1 surface level and function and suggest new drug targets for the treatment of neurological disorders.

Indexed as

Cell biologyCellular neuroscienceMolecular biologyMolecular neuroscience

Identifiers

PMID35434559
PMCPMC9010640
OpenAlexW4221123158

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.