Evidence map›Paper›PMID 35433971›Full record

ArticleAnnals of translational medicine2022

Integrative analysis of key microRNA-mRNA complexes and pathways in aortic aneurysm.

Yufei Fu, Yingying Mao, Ruoshi Chen, Anfeng Yu, Yifei Yin, Xi Wang, Liang Ma, Xin Chen

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of translational medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yufei FuKey Laboratory of Digestive Pathophysiology of Zhejiang Province, The First Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang Chinese Medical University, Hangzhou, China.
Yingying MaoDepartment of Epidemiology and Biostatistics, Zhejiang Chinese Medical University, Hangzhou, China.
Ruoshi ChenDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Zhejiang University, Medical School of Zhejiang University, Hangzhou, China.
Anfeng YuDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Zhejiang University, Medical School of Zhejiang University, Hangzhou, China.
Yifei YinKey Laboratory of Digestive Pathophysiology of Zhejiang Province, The First Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang Chinese Medical University, Hangzhou, China.
Xi WangKey Laboratory of Digestive Pathophysiology of Zhejiang Province, The First Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang Chinese Medical University, Hangzhou, China.
Liang MaDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Zhejiang University, Medical School of Zhejiang University, Hangzhou, China.
Xin ChenDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Zhejiang University, Medical School of Zhejiang University, Hangzhou, China.
Zhejiang Chinese Medical University · CNZhejiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: This study investigated the therapeutic targets of aortic aneurysm (AA) and provided insights into the pathogenesis and molecular mechanisms of AA. Methods: The messenger RNA (mRNA) datasets, GSE9106 (blood samples) and GSE7084 (tissue samples), and the microRNA (miRNA) datasets, GSE92427 (blood samples) and GSE110527 (tissue samples), were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) and differentially expressed miRNAs (DE-miRNAs) were analyzed by limma. Based on the co-DEGs and co-DE-miRNAs between the AA blood and tissue datasets, the miRNA-mRNA regulatory pairs were predicted. Functional enrichment analyses and gene set enrichment analysis (GSEA) were performed and the protein-protein interaction (PPI) network was generated to further analyze the related genes and their functions. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and tyramide signal amplification (TSA)-in situ hybridization (ISH) assays were performed to detect the expression of co-DE-miRNAs in AA clinical tissue samples and normal aorta samples. Results: There were 19 upregulated and 5 downregulated co-differential mRNAs. Conclusions: This study provided evidence regarding the differential regulatory miRNA-mRNA networks in AA blood and tissue samples and identified key genes and signaling pathways related to AA, which provided insights into potential targets and mechanisms of AA pathogenesis and progression.

Indexed as

Aortic aneurysm (AA)gene set enrichment analysis (GSEA)integrated analysismicroRNA-messenger RNA complex (miRNA-mRNA complex)tyramide signal amplification-in situ hybridization assay (TSA-ISH assay)

Identifiers

PMID35433971
PMCPMC9011231
OpenAlexW4224233326

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.