Evidence map›Paper›PMID 35433736›Full record

ArticleFrontiers in medicine2022

Antibiotic Therapy and Vaginal Microbiota Transplantation Reduce Endometriosis Disease Progression in Female Mice

Feilei Lu, Jing Wei, Yanying Zhong, Ying Feng, Bo Ma, Yifei Xiong, Kehong Wei, Buzhen Tan, Tingtao Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 1 pooled it
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.

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  11. mSystems · 2025
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  17. Reproductive tract microbiome dysbiosis associated with gynecological diseases.Frontiers in cellular and infection microbiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Feilei LuDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
Jing WeiInstitute of Translational Medicine, Nanchang University, Nanchang, China.
Yanying ZhongDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
Ying FengDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
Bo MaDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
Yifei XiongDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
Kehong WeiQueen Mary School, Nanchang University, Nanchang, China.
Buzhen TanDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
Tingtao ChenInstitute of Translational Medicine, Nanchang University, Nanchang, China.
Nanchang University · CNSecond Affiliated Hospital of Nanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endometriosis (EMS) is a disease characterized by estrogen-dependent, chronic inflammatory, and annoying symptoms, which inflicts about 10% reproductive-age women. The diagnosis of endometriosis mainly depends on pathological examination after surgical resection while the pathogenesis of EMS is not clear enough. Surgical resection and drug therapy (including painkillers and hormone therapy, especially gonadotropin-releasing hormone analogs, GnRH-a) are widely used, but they are expensive and have many side effects. There are few studies on vaginal microorganisms in women with endometriosis. We collected vaginal secretions from women with EMS confirmed by pathology and demonstrated that they were different from that of healthy women by 16s rRNA high-throughput sequencing. Additionally, we established the EMS model in female mice by intraperitoneally injecting fragments from donor mice (3-week growth). Then, the mice were treated with mixed antibiotics (vagina) and NF-κB signaling pathway inhibitors (intraperitoneal injection), respectively. The result suggested that the ectopic lesions were inhibited. In addition, inflammatory cytokines IL-1β, IL-6, and TNF-α in peritoneal fluid, cell proliferation marker ki-67, and macrophage marker Iba-1 in ectopic lesions decreased significantly from that of mock mice. We also observed similar results as above by vaginal microbiota transplantation (VMT) and subcutaneous injection of leuprorelin acetate (LA, one of GnRH-a) for mice with EMS. These results showed that vaginal use of antibiotics or VMT is helpful to treat endometriosis in mice. However, due to the great difference between human and mouse vaginal microbiota, its mechanism and clinical transformation application still need to be further studied in the future.

Indexed as

endometriosishigh-throughput sequencingNF-κB signaling pathwaypathogenesisvaginal microbiota

Identifiers

PMID35433736
PMCPMC9005645
OpenAlexW4221003332

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.