ArticleThe Journal of infectious diseases2022
Amplicon Sequencing as a Potential Surveillance Tool for Complexity of Infection and Drug Resistance Markers in Plasmodium falciparum Asymptomatic Infections.
Article in The Journal of infectious diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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17 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Genetic diversity, population structure, and drug resistance of Plasmodium falciparum in Kenya: a systematic review and meta-analysis.Malaria journal · 2025Pooled it
- UnderstandingiScience · 2026Article
- Article
- Characterizing Plasmodium falciparum genetic diversity and complexity of infections in clinical malaria infections in Western and Coastal Kenya using the poly-alpha microsatellite marker.Malaria journal · 2025Article
- UnderstandingmedRxiv : the preprint server for health sciences · 2025Article
- Detection of novelMicrobial genomics · 2025Article
- Stable Plasmodium falciparum merozoite surface protein-1 allelic diversity despite decreasing parasitaemia in children with multiple malaria infections.Malaria journal · 2025Article
- Detection of low frequency artemisinin resistance mutations, C469Y, P553L and A675V, and fixed antifolate resistance mutations in asymptomatic primary school children in Kenya.BMC infectious diseases · 2025Article
- Automated total nucleic acid extraction with magnetic beads for the detection of Plasmodium falciparum in large study cohorts.Malaria journal · 2024Article
- Integrating targeted genetic markers to genotyping-by-sequencing for an ultimate genotyping tool.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- A retrospective analysis ofAntimicrobial agents and chemotherapy · 2024Article
- Lineage-informative microhaplotypes for recurrence classification and spatio-temporal surveillance of Plasmodium vivax malaria parasites.Nature communications · 2024Article
- Distinct transcriptomic signatures define febrile malaria depending on initial infective states, asymptomatic or uninfected.BMC infectious diseases · 2024Article
- ONT sequencing identifies a high prevalence ofFrontiers in genetics · 2024Article
- Comparing newly developed SNP barcode panels with microsatellites to explore population genetics of malaria parasites in the Peruvian Amazon.Frontiers in genetics · 2024Article
- Drug resistance and vaccine target surveillance of Plasmodium falciparum using nanopore sequencing in Ghana.Nature microbiology · 2023Article
- Population structure and genetic connectivity ofFrontiers in epidemiology · 2023Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundGenotyping Plasmodium falciparum subpopulations in malaria infections is an important aspect of malaria molecular epidemiology to understand within-host diversity and the frequency of drug resistance markers.
methodsWe characterized P. falciparum genetic diversity in asymptomatic infections and subsequent first febrile infections using amplicon sequencing (AmpSeq) of ama1 in Coastal Kenya. We also examined temporal changes in haplotype frequencies of mdr1, a drug-resistant marker.
resultsWe found >60% of the infections were polyclonal (complexity of infection [COI] >1) and there was a reduction in COI over time. Asymptomatic infections had a significantly higher mean COI than febrile infections based on ama1 sequences (2.7 [95% confidence interval {CI}, 2.65-2.77] vs 2.22 [95% CI, 2.17-2.29], respectively). Moreover, an analysis of 30 paired asymptomatic and first febrile infections revealed that many first febrile infections (91%) were due to the presence of new ama1 haplotypes. The mdr1-YY haplotype, associated with chloroquine and amodiaquine resistance, decreased over time, while the NY (wild type) and the NF (modulates response to lumefantrine) haplotypes increased.
conclusionsThis study emphasizes the utility of AmpSeq in characterizing parasite diversity as it can determine relative proportions of clones and detect minority clones. The usefulness of AmpSeq in antimalarial drug resistance surveillance is also highlighted.
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