Evidence map›Paper›PMID 35429303›Full record

ReviewBiotechnology letters2022

Production of phenylpyruvic acid by engineered L-amino acid deaminase from Proteus mirabilis.

Jia Liu, Jianmin Liu, Bin Yang, Cong Gao, Wei Song, Guipeng Hu, Liming Liu, Jing Wu

Abstract readReview
PubMed Publisher
In one paragraph

Review in Biotechnology letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. EngineeringRSC advances · 2024
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jia LiuState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Jianmin LiuShandong Huishilai Biotechnology Co., Ltd, Jinan, 250098, China.
Bin YangState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Cong GaoState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Wei SongSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Guipeng HuSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Liming LiuState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Jing WuSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China. wujing@jiangnan.edu.cn.
Jiangnan University · CNState Key Laboratory of Food Science and Technology · CN

Funding

Key Technologies Research and Development Program 2021YFC2100100National First-class Discipline Program of Light Industry Technology and Engineering LITE2018-08National Natural Science Foundation of China 22008089
6 · The paper itself

Abstract

objectivesThis study aimed to develop an efficient enzymatic strategy for the industrial production of phenylpyruvate (PPA) from L-phenylpyruvic acid (L-Phe).

resultsL-amino acid deaminase from Proteus mirabilis was expressed in Escherichia coli BL21 (DE3) and modified to release product inhibition by employing conformational dynamics engineering. Based on structural analysis, two residues (E145/L341) were identified for reducing interactions between the product and enzyme and increasing flexibility of the protein, thereby facilitating the product release. The mutant M2

conclusionsThe engineered catalyst can significantly reduce product inhibition and facilitate the effective industrial synthesis of PPA.

Indexed as

Amino AcidsProteus mirabilisEscherichia coliPhenylpyruvic AcidsAmino Acidsphenylpyruvic acidPhenylpyruvic AcidsBio-catalysisL-amino acid deaminasePhenylpyruvateProduct inhibitionProtein engineering

Identifiers

PMID35429303
OpenAlexW4226038250

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.