Evidence map›Paper›PMID 35427794›Full record

ArticleBiochimica et biophysica acta. Molecular and cell biology of lipids2022

Ribosomal targeting strategy and nuclear labeling to analyze photoreceptor phosphoinositide signatures.

Ammaji Rajala, Rahul Rajala, Kenneth Teel, Raju V S Rajala

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta. Molecular and cell biology of lipids, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Ammaji RajalaDepartment of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Dean McGee Eye Institute, Oklahoma City, OK 73014, USA.
Rahul RajalaDepartment of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Cardiovascular Biology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73014, USA.
Kenneth TeelDepartment of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Dean McGee Eye Institute, Oklahoma City, OK 73014, USA.
Raju V S RajalaDepartment of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Dean McGee Eye Institute, Oklahoma City, OK 73014, USA. Electronic address: raju-rajala@ouhsc.edu.
Oklahoma Medical Research Foundation · USDean McGee Eye Institute · US

Funding

P30-CENTER CORE GRANT FOR VISION RESEARCHP30EY021725 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI Michelle C Callegan · 2011 to 2026
$10.5M
SECOND MESSENGERS IN THE RETINAR01EY000871 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI RAJALA, RAJU VS · 1985 to 2020
$7.2M
CORE--MOLECULAR BIOLOGYP30EY012190 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI ANDERSON, ROBERT E · 1999 to 2009
$5.2M
Neuroprotection Mechanism for PhotoreceptorsR01EY030024 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI RAJALA, RAJU VS · 2019 to 2022
$1.7M
NEI NIH HHS P30 EY012190NEI NIH HHS P30 EY021725NEI NIH HHS R01 EY000871NEI NIH HHS R01 EY030024
6 · The paper itself

Abstract

Reversible phosphorylation of phosphatidylinositol by phosphoinositide (PI) kinases and phosphatases generates seven distinct phosphoinositide phosphates, called phosphoinositides or PIPs. All seven PIPs are formed in the retina and photoreceptor cells. Around 50 genes in the mammalian genome encode PI kinases and PI phosphatases. There are no studies available on the distribution of these enzymes in the retina and photoreceptors.

aimTo employ Ribosomal Targeting Strategy and Nuclear Labeling to Analyze Phosphoinositide Signatures in rod-photoreceptor cells.

methodsHA-tagging of ribosomal protein Rpl22 was induced with Cre-recombinase under the control of the rhodopsin promoter. Actively translating mRNAs associated with polyribosomes were isolated by immunoprecipitation with HA antibody, followed by RNA isolation and gene identification. We also isolated biotinylated-rod nuclei from NuTRAP mice under the control of the rhodopsin-Cre promoter and analyzed nuclear phosphoinositides.

resultsOur results indicate that the expression of class I and class III PI 3-kinase, PI4K IIIβ, PI 5-kinase, PIKfyve, PI3-phosphatases, MTMR2, 4, 6, 7, 14, PI4-phosphatase, TMEM55A, PI 5-phosphatases, SYNJI, INPP5B, INPP5E, INPP5F, SKIP and other phosphatases with dual substrate specificity, PTPMT1, SCAM1, and FIG4 are highly enriched in rod photoreceptor cells compared with the retina and cone-like retina. Our analysis identified the presence of PI(4)P, PI(3,4)P

conclusionsOur studies for the first time demonstrate the expression of PI kinases, PI phosphatases, and nuclear PIPs in rod photoreceptor cells. The NuTRAP mice may be useful not only for epigenetic and transcriptomic studies but also for in vivo cell-specific lipidomics research.

Indexed as

Phosphoric Monoester HydrolasesPhotoreceptor CellsRibosomes1-Phosphatidylinositol 4-KinaseAnimalsFlavoproteinsMicePhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhosphoinositide 5-PhosphatasesPhosphoinositide PhosphatasesProtein Tyrosine Phosphatases, Non-ReceptorRhodopsin1-Phosphatidylinositol 4-KinaseFig4 protein, mouseFlavoproteinsMtmr2 protein, mousePhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhosphoinositide 5-PhosphatasesPhosphoinositide PhosphatasesPhosphoric Monoester HydrolasesPikfyve protein, mouseProtein Tyrosine Phosphatases, Non-ReceptorRhodopsinActively translating mRNAsNuclear phosphoinositidesPhosphoinositide kinasesPhosphoinositide phosphatasesPhosphoinositidesPhotoreceptor cellsRetinaRetinal degeneration

Identifiers

PMID35427794
PMCPMC10812878
OpenAlexW4223633520

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.