Evidence map›Paper›PMID 35427452›Full record

ArticleACS sensors2022

Tuning the Sensitivity of Genetically Encoded Fluorescent Potassium Indicators through Structure-Guided and Genome Mining Strategies.

Cristina C Torres Cabán, Minghan Yang, Cuixin Lai, Lina Yang, Fedor V Subach, Brian O Smith, Kiryl D Piatkevich, Edward S Boyden

Open access · bronzeAbstract read
In one paragraph

Article in ACS sensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
4.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 37 citations in OpenAlex.

  1. Localizable Fluorescent Metal Ion Indicators With Tunable Colors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Article
  4. Neurophotonics · 2025
    Article
  5. Article
  6. Review
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  8. Article
  9. Article
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  13. Article
  14. Genetically encoded fluorescent sensors for metals in biology.Current opinion in chemical biology · 2023
    Review
  15. Review
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 4 countries.

Cristina C Torres CabánMcGovern Institute for Brain Research, MIT, Cambridge, Massachusetts 02139, United States.
Minghan YangSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.
Cuixin LaiSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.
Lina YangSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.
Fedor V SubachComplex of NBICS Technologies, National Research Center "Kurchatov Institute", Moscow 123182, Russia.ORCID 0000-0003-2720-7821
Brian O SmithInstitute of Molecular, Cell & Systems Biology, College of Medical Veterinary & Life Sciences, University of Glasgow, Glasgow G128QQ, United Kingdom.
Kiryl D PiatkevichSchool of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.ORCID 0000-0002-7777-9468
Edward S BoydenMcGovern Institute for Brain Research, MIT, Cambridge, Massachusetts 02139, United States.
Westlake University · CNHoward Hughes Medical Institute · USJilin University · CNKurchatov Institute · RUMcGovern Institute for Brain Research · USUniversity of Glasgow · GB

Funding

Novel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7M
Redesigning General Anesthesia (Admin Supp)R01GM104948 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., BROWN, EMERY N · 2012 to 2016
$4.4M
Voltage Imaging of Astrocyte-Neuron InteractionsR01NS113499 · NINDS · TUFTS UNIVERSITY BOSTON · PI DULLA, CHRIS G · 2019 to 2023
$3.7M
High-Performance Imaging Through Scattering Living TissueR01DA045549 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2020
$3.5M
Multidimensional Optimization of Voltage Indicators for In Vivo Neural Activity ImagingR01MH122971 · NIMH · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI HAN, XUE · 2020 to 2024
$3.4M
RNA Scaffolds for Cell Specific Multiplexed Neural ObservationR01MH114031 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2020
$2.8M
High throughput assaying of circuit activity and connectivity in brain organoidsRF1MH123977 · NIMH · HARVARD UNIVERSITY · PI ARLOTTA, PAOLA, BOYDEN, EDWARD S. · 2020 to 2020
$2.5M
Targeted regulation of transcript stability through RNA methylation and intron retentionR01GM123977 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GOODARZI, HANI · 2017 to 2021
$2.0M
Opto-Crown: Transparent skulls with embedded optics for cortex-wide cellular resolution imaging in freely moving miceRF1NS113287 · NINDS · UNIVERSITY OF MINNESOTA · PI BOYDEN, EDWARD S., KODANDARAMAIAH, SUHASA B · 2019 to 2019
$2.0M
Novel tools for spatiotemporal modulation of astrocytes in neuronal circuitsRF1DA049005 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI SUR, MRIGANKA · 2019 to 2019
$1.5M
High Speed, Multi-sensor Light Field Deconvolution Microscopy for Whole Brain Recording of Neuronal ActivityR43MH109332 · NIMH · LEAFLABS, LLC · PI BOYDEN, EDWARD S., SHERWOOD, JOHN L · 2016 to 2017
$892k
NIDA NIH HHS R01 DA029639NIDA NIH HHS R01 DA045549NIDA NIH HHS RF1 DA049005NIGMS NIH HHS R01 GM104948NIGMS NIH HHS R01 GM123977NIMH NIH HHS R01 MH114031NIMH NIH HHS R01 MH122971NIMH NIH HHS R43 MH109332NIMH NIH HHS RF1 MH123977NINDS NIH HHS R01 NS113499NINDS NIH HHS RF1 NS113287
6 · The paper itself

Abstract

Genetically encoded potassium indicators lack optimal binding affinity for monitoring intracellular dynamics in mammalian cells. Through structure-guided design and genome mining of potassium binding proteins, we developed green fluorescent potassium indicators with a broad range of binding affinities. KRaION1 (K

Indexed as

Escherichia coliPotassiumAnimalsCarrier ProteinsGreen Fluorescent ProteinsHeLa CellsHumansMammalsOptical ImagingCarrier ProteinsGreen Fluorescent ProteinsPotassiumfluorescent proteingenome miningNMR structurepotassium sensorprotein engineeringrational design

Identifiers

PMID35427452
PMCPMC9150168
OpenAlexW4225794806

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.