ReviewWorld journal of microbiology & biotechnology2022
Recent advances in the microbial production of squalene.
Review in World journal of microbiology & biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Research progress on metabolic engineering for efficient squalene synthesis in yeast cell factories.Biotechnology letters · 2026Review
- Effects of Dietary Squalene Supplementation on the Growth Performance and Disease Resistance of Largemouth Bass.Veterinary sciences · 2026Article
- De novo biosynthesis of Asperosaponin VI inSynthetic and systems biotechnology · 2025Article
- Article
- Production of squalene and fatty acids by Thraustochytrium sp. RT2316-16: effects of dissolved oxygen and the medium composition.Bioresources and bioprocessing · 2025Article
- The Sustainable Production of Terpenoids in Cyanobacterial Chassis.Microorganisms · 2025Review
- A Biosynthetic and Taxonomic Atlas of the Global Lichen Holobiont.Environmental microbiology · 2025Article
- Diverse lifestyles and adaptive evolution of uncultured UBA5794 actinobacteria, a sister order of "Candidatus actinomarinales".Environmental microbiome · 2025Article
- Effect of Terroir on Phenolic Content and Aroma Properties of Grapes and Wines.Foods (Basel, Switzerland) · 2025Article
- Optimization of Squalene Production byMolecules (Basel, Switzerland) · 2025Article
- Overexpression of tRNA mFrontiers in microbiology · 2025Article
- Analytical Determination of Squalene in Extra Virgin Olive Oil and Olive Processing By-Products, and Its Valorization as an Ingredient in Functional Food-A Critical Review.Molecules (Basel, Switzerland) · 2024Review
- Microbial Squalene: A Sustainable Alternative for the Cosmetics and Pharmaceutical Industry - A Review.Engineering in life sciences · 2024Review
- Sustainable biosynthesis of squalene from waste cooking oil by the yeastMetabolic engineering communications · 2024Article
- Efforts toward Ambergris Biosynthesis.Chem & bio engineering · 2024Review
- Natural Products Biosynthesis byAntibiotics (Basel, Switzerland) · 2024Article
- Engineering peroxisomal biosynthetic pathways for maximization of triterpene production inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Anti-Infection of Oral Microorganisms from Herbal Medicine ofDrug design, development and therapy · 2024Review
- Bioprocess conditions and regulation factors to optimize squalene production in thraustochytrids.World journal of microbiology & biotechnology · 2023Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Squalene is a triterpene hydrocarbon, a biochemical precursor for all steroids in plants and animals. It is a principal component of human surface lipids, in particular of sebum. Squalene has several applications in the food, pharmaceutical, and medical sectors. It is essentially used as a dietary supplement, vaccine adjuvant, moisturizer, cardio-protective agent, anti-tumor agent and natural antioxidant. With the increased demand for squalene along with regulations on shark-derived squalene, there is a need to find alternatives for squalene production which are low-cost as well as sustainable. Microbial platforms are being considered as a potential option to meet such challenges. Considerable progress has been made using both wild-type and engineered microbial strains for improved productivity and yields of squalene. Native strains for squalene production are usually limited by low growth rates and lesser titers. Metabolic engineering, which is a rational strain engineering tool, has enabled the development of microbial strains such as Saccharomyces cerevisiae and Yarrowia lipolytica, to overproduce the squalene in high titers. This review focuses on key strain engineering strategies involving both in-silico and in-vitro techniques. Emphasis is made on gene manipulations for improved precursor pool, enzyme modifications, cofactor regeneration, up-regulation of limiting reactions, and downregulation of competing reactions during squalene production. Process strategies and challenges related to both upstream and downstream during mass cultivation are detailed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.