ArticleEnvironmental monitoring and assessment2022
Evaluation of crAssphage as a human-specific microbial source-tracking marker in the Republic of Korea.
Article in Environmental monitoring and assessment, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- CrAssphage as a Human Enteric Viral Contamination Bioindicator in Marketed Bivalve Mollusks.Viruses · 2025Article
- Evaluation of crAssphages as a potential marker of human viral contamination in environmental water and fresh leafy greens.Frontiers in microbiology · 2024Article
- Tools and methodology toFrontiers in microbiology · 2024Review
- CrAssphage May Be Viable Markers of Contamination in Pristine and Contaminated River Water.mSystems · 2023Article
- Improvement of crAssphage detection/quantification method and its extensive application for food safety.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
CrAssphage is a novel and by far the most abundant bacteriophage in the human gut and has been proposed as a human-specific microbial source tracking (MST) marker. However, its global use as a human-specific MST marker requires validation in more extensive regions. The purpose of this study was to evaluate the specificity and abundance of the human-specific MST marker crAssphage with PCR and RT-PCR assays in human and animal feces in Korea. The prevalence of crAssphage was confirmed in 94 human feces samples (subjects: 19 to 45 years old) and 56 animal feces samples (from birds, raccoons, squirrels, weasels, deer, wild boars, hares, cats, and dogs). CrAssphage showed sensitivity of 0.39 and specificity of 1.00 in Korea, with a sequencing analysis showing that genotype II was dominant at 71.9%. The quantitative analysis showed that crAssphage is sufficiently abundant in human feces given the high concentration range of 4.26 to 8.25 log gene copies (GC)/ng in human feces. In conclusion, this study confirmed the crAssphage as a specific and abundant MST marker with which to identify human fecal contamination in Korea.
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