Evidence map›Paper›PMID 35426036›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2022

FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition.

Erika P New, Nihan Semerci, Asli Ozmen, Xiaofang Guo, Venkata A Jonnalagadda, Joung Woul Kim, Matthew L Anderson, Ozlem Guzeloglu-Kayisli, Anthony N Imudia, Charles J Lockwood and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Regulation of androgen receptor stability by the βFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Erika P NewDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Nihan SemerciDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Asli OzmenDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Xiaofang GuoDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Venkata A JonnalagaddaDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Joung Woul KimDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Matthew L AndersonDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Ozlem Guzeloglu-KayisliDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Anthony N ImudiaDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Charles J LockwoodDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA.
Umit A KayisliDepartment of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, 560 Channelside Drive, Office MDD738, Tampa, FL, 33602, USA. uakayisli@usf.edu.
University of South Florida · USShady Grove Fertility Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The FK506-binding protein 51 (FKBP51) binds progesterone receptor (PR), glucocorticoid receptor (GR), and androgen receptor (AR) to coregulate their transcriptional activity. We evaluated FKBP51 expression and function in human leiomyoma vs. myometrial tissues and primary cultures to discover FKBP51 role(s) in the pathogenesis of leiomyomas. Quantification of in situ FKBP51 mRNA and protein levels inpaired myometrial vs. leiomyoma tissues from proliferative and secretory phases were analyzed by qPCR (n = 14), immunoblotting (n = 20), and immunohistochemistry (n = 12). Control (scramble) vs. FKBP5 siRNA-transfected leiomyoma cell cultures were assessed for proliferation, apoptosis, and mRNA levels of genes involved in cell survival and extracellular matrix (ECM) formation. Significantly higher FKBP5 mRNA levels were detected in leiomyoma vs. paired myometrium (P < 0.001). Immunoblot (P = 0.001) and immunostaining (P ≤ 0.001) confirmed increased FKBP51 levels in leiomyoma vs. paired myometrium. Compared to control siRNA transfection, FKBP5-silenced leiomyoma cell cultures displayed significantly decreased cell survival factors and reduced proliferation (P < 0.05). Moreover, qPCR analysis revealed significantly lower mRNA levels of ECM, TIPM1, and TIPM3 proteins in FKBP5-silenced leiomyoma cell cultures (P < 0.05). Increased FKBP51 expression in leiomyoma likely involves dysregulation of steroid signaling by blocking GR and PR action and promoting proliferation and ECM production. Evaluating the effect of FKBP51 inhibition in preclinical studies will clarify its significance as a potential therapeutic approach against leiomyoma.

Indexed as

LeiomyomaTacrolimus Binding ProteinsUterine NeoplasmsCell ProliferationExtracellular MatrixFemaleHumansImmunohistochemistryMyometriumRNA, MessengerTacrolimus Binding Protein 5RNA, MessengerTacrolimus Binding Protein 5Tacrolimus Binding ProteinsExtracellular matrixProgesterone antagonistSteroid receptor signalingUterine fibroidUterine leiomyomas

Identifiers

PMID35426036
PMCPMC9009985
OpenAlexW4223952533

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.