Evidence map›Paper›PMID 35424416›Full record

ArticleRSC advances2021

Quantifying genome DNA during whole-genome amplification

Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu

Abstract read
In one paragraph

Article in RSC advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jing TuState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing 210096 China jtu@seu.edu.cn +86-25-83792396.ORCID https://orcid.org/0000-0002-1310-5915
Yi QiaoState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing 210096 China jtu@seu.edu.cn +86-25-83792396.ORCID https://orcid.org/0000-0002-1371-6775
Yuhan LuoState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing 210096 China jtu@seu.edu.cn +86-25-83792396.
Naiyun LongState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing 210096 China jtu@seu.edu.cn +86-25-83792396.ORCID https://orcid.org/0000-0001-8696-2814
Zuhong LuState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing 210096 China jtu@seu.edu.cn +86-25-83792396.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA quantification is important in the research of life sciences. In an independent quantification process, the extracted part of a DNA sample is usually difficult to be recycled for further use while the widely used real-time PCR is used to count the copies with certain sequences. Based on the popular multiple displacement amplification (MDA), we proposed and performed quantitative real-time MDA to obtain the information of template amount based on fluorescence signals while amplifying whole-genome DNA. The detection limit of real-time MDA was as low as 0.5 pg μl

Identifiers

PMID35424416
PMCPMC8694541

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.