ArticleBiosensors & bioelectronics2022
Split T7 promoter-based isothermal transcription amplification for one-step fluorescence detection of SARS-CoV-2 and emerging variants.
Article in Biosensors & bioelectronics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 61 citations in OpenAlex.
- Base gap switches as transcriptional regulators and their applications as biosensors.Nature sensors · 2026Article
- A Modular T7 RNA Polymerase Toolbox Linking Selective miRNA Detection to Signal Amplification and Protein Expression.Journal of the American Chemical Society · 2025Article
- Current biosensing strategies based on in vitro T7 RNA polymerase reaction.Biotechnology notes (Amsterdam, Netherlands) · 2025Review
- Engineering Gene-Specific DNAzymes for Accessible and Multiplexed Nucleic Acid Testing.JACS Au · 2024Article
- Article
- Tunable T7 Promoter Orthogonality on T7RNAP forACS synthetic biology · 2023Article
- Label-Free Detection of T4 Polynucleotide Kinase Activity and Inhibition via Malachite Green Aptamer Generated from Ligation-Triggered Transcription.Biosensors · 2023Article
- Low-Temperature Loop-Mediated Isothermal Amplification Operating at Physiological Temperature.Biosensors · 2023Article
- Review
- Review
- Article
- Easy Express Extraction (TripleMicroorganisms · 2022Article
- Article
- Single-tube isothermal label-free fluorescent sensor for pathogen detection based on genetic signatures.Frontiers in chemistry · 2022Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The negative global impact of the coronavirus disease pandemic has highlighted the crucial need for a rapid and convenient method of viral RNA detection. In this study, we report a novel method, termed as the split T7 promoter-based isothermal transcription amplification with light-up RNA aptamer (STAR), for one-pot detection of viral RNA. STAR uses a split T7 promoter that is applied to a three-way junction to mediate the selective transcription by the T7 RNA polymerase in the presence of target RNA. In addition, a light-up RNA aptamer is used for signal amplification. STAR can detect viral RNA in less than 30 min with high specificity and sensitivity. By testing of 60 nasopharyngeal SARS-CoV-2 samples, the STAR assay demonstrates an excellent sensitivity and specificity of 96.7% and 100%, respectively. Moreover, we provide experimental evidence of the broad applicability of this assay through the multiplex detection of SARS-CoV-2 variants (D614G mutation) and direct detection of bacterial 16S rRNA.
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