ArticleMicrobial biotechnology2022
Novel protein carrier system based on cyanobacterial nano-sized extracellular vesicles for application in fish.
Article in Microbial biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- RpaA Overexpression Enhances Bioluminescence Intensity and Elevates Rhythmic Extracellular Vesicle Yield inLife (Basel, Switzerland) · 2026Article
- Bacterial Extracellular Vesicles in Biomedical Research and Clinical Translation.Microbial biotechnology · 2026Review
- Cyanobacterial Extracellular Vesicles as Protein Carriers: Towards Fish Vaccination.Microbial biotechnology · 2026Article
- Rhamnose biosynthesis is not impaired by the deletion of putativeApplied and environmental microbiology · 2025Article
- Exploring the Proteomic Landscape and Immunomodulatory Functions ofJournal of microbiology and biotechnology · 2024Article
- Characterization of Spirulina-derived extracellular vesicles and their potential as a vaccine adjuvant.Journal of extracellular biology · 2024Article
- EXCRETE workflow enables deep proteomics of the microbial extracellular environment.Communications biology · 2024Article
- Biomedical engineering utilizing living photosynthetic cyanobacteria and microalgae: Current status and future prospects.Materials today. Bio · 2024Review
- Immunomodulatory properties ofFrontiers in immunology · 2024Article
- LPS-Induced Mortality in Zebrafish: Preliminary Characterisation of Common Fish Pathogens.Microorganisms · 2023Article
- Incorporation, fate, and turnover of free fatty acids in cyanobacteria.FEMS microbiology reviews · 2023Review
- Emerging Trends of Nanotechnology and Genetic Engineering in Cyanobacteria to Optimize Production for Future Applications.Life (Basel, Switzerland) · 2022Review
- Novel protein carrier system based on cyanobacterial nano-sized extracellular vesicles for application in fish.Microbial biotechnology · 2022Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aquaculture has been one of the fastest-growing food industry sectors, expanding at the pace of consumers' demands. To promote safe and effective fish growth performance strategies, and to stimulate environmentally friendly solutions to protect fish against disease outbreaks, new approaches are needed to safeguard fish welfare, as well as farmers and consumers interests. Here, we tested the use of cyanobacterial extracellular vesicles (EVs) as a novel nanocarrier system of heterologous proteins for applications in fish. We started by incubating zebrafish larvae with Synechocystis sp. PCC6803 EVs, isolated from selected mutant strains with different cell envelope characteristics. Results show that Synechocystis EVs are biocompatible with fish larvae, regardless of their structural composition, as EVs neither induced fish mortality nor triggered significant inflammatory responses. We establish also that cyanobacteria are amenable to engineering heterologous protein expression and loading into EVs, for which we used the reporter sfGFP. Moreover, upon immersion treatment, we successfully demonstrate that sfGFP-loaded Synechocystis EVs accumulate in the gastrointestinal tract of zebrafish larvae. This work opens the possibility of using cyanobacterial EVs as a novel biotechnological tool in fish, with prospective applications in carrying proteins/enzymes, for example for modulating their nutritional status or stimulating specific adaptive immune responses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.