ArticleFrontiers in veterinary science2022
Efficacy of Tricaine (MS-222) and Hypothermia as Anesthetic Agents for Blocking Sensorimotor Responses in Larval Zebrafish.
Article in Frontiers in veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 33 citations in OpenAlex.
- Evolution and Reorganization of the Hierarchical Brain-wide Neuronal Network Through Anesthesia.Neuroscience bulletin · 2026Article
- Protocol for live-imaging of blood flow and vascular permeability in zebrafish larvae.STAR protocols · 2026Article
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- Toxic effects of biodegradable polylactic acid nanoplastics on developing zebrafish (Danio rerio).Scientific reports · 2025Article
- EMB is essential for enteric nervous system development mediated by PI3K signaling.Genome medicine · 2025Article
- SG06, a Chalcone Derivative Targets PI3K/AKT1 Pathway for Neuroprotection and Cognitive Enhancement in an Alzheimer's Disease-Like Zebrafish Model.Molecular neurobiology · 2025Article
- Thymol and menthol as anaesthetics for short transportation of zebrafish larva.Fish physiology and biochemistry · 2025Article
- Inhibition of inner ear macrophage phagocytosis alleviates cisplatin-induced ototoxicity.Communications biology · 2025Article
- Behavioral and neurophysiological effects of electrical stunning on zebrafish larvae.Lab animal · 2025Article
- Chronotropic and vasoactive properties of the gut bacterial short-chain fatty acids in larval zebrafish.Physiological genomics · 2024Article
- Per- and Polyfluoroalkyl Substances: Impacts on Morphology, Behavior and Lipid Levels in Zebrafish Embryos.Toxics · 2024Article
- Functional neuronal circuits emerge in the absence of developmental activity.Nature communications · 2024Article
- Article
- Assessment of the effect of tricaine (MS-222)-induced anesthesia on brain-wide neuronal activity of zebrafish (Frontiers in neuroscience · 2024Article
- Review
- A New Bioassay for the Detection of Paralytic and Amnesic Biotoxins Based on Motor Behavior Impairments of Zebrafish Larvae.International journal of molecular sciences · 2023Article
- Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Tricaine, or MS-222, is the most commonly used chemical anesthetic in zebrafish research. It is thought to act via blocking voltage-gated sodium channels, though its mechanism of action, particularly at the neuronal level, is not yet fully understood. Here, we first characterized the effects of tricaine on both body balance and touch responses in freely swimming animals, before determining its effect on the neural activity underlying the optokinetic response at the level of motion perception, sensorimotor signaling and the generation of behavior in immobilized animals. We found that the standard dose for larvae (168 mg/L) induced loss of righting reflex within 30 seconds, which then recovered within 3 minutes. Optokinetic behavior recovered within 15 minutes. Calcium imaging showed that tricaine interferes with optokinetic behavior by interruption of the signals between the pretectum and hindbrain. The motion sensitivity indices of identified sensory neurons were unchanged in larvae exposed to tricaine, though fewer such neurons were detected, leaving a small population of active sensory neurons. We then compared tricaine with gradual cooling, a potential non-chemical alternative method of anesthesia. While neuronal tuning appeared to be affected in a similar manner during gradual cooling, gradual cooling induced a surge in calcium levels in both the pretectum and hindbrain. This calcium surge, alongside a drop in heartrate, is potentially associated with harmful changes in physiology and suggests that tricaine is a better anesthetic agent than gradual cooling for zebrafish laboratory research.
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