ReviewFrontiers in bioengineering and biotechnology2022
The Challenges in Developing Efficient and Robust Synthetic Homing Endonuclease Gene Drives.
Review in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The nanosd integral gene drive enables population modification of the malaria vector Anopheles gambiae.G3 (Bethesda, Md.) · 2026Article
- Finding the perfect promoter for Cas9 in homing gene drives using single cell transcriptome data.Nature communications · 2026Article
- A simulation-based deep learning framework for spatially explicit malaria modeling of CRISPR suppression gene drive mosquitoes.iScience · 2026Article
- Sex distorter male drive for resistance-resilient population control of the human malaria vector Anopheles gambiae.Nature communications · 2026Article
- Leveraging sex determination systems for genetic biocontrol of dipteran pests.Current opinion in insect science · 2026Review
- A Comparative Assessment of Self-limiting Genetic Control Strategies for Population Suppression.Molecular biology and evolution · 2025Article
- Population suppression by release of insects carrying a dominant sterile homing gene drive targeting doublesex in Drosophila.Nature communications · 2024Article
- Arthropod promoters for genetic control of disease vectors.Trends in parasitology · 2024Review
- Performance characteristics allow for confinement of a CRISPR toxin-antidote gene drive for population suppression in a reaction-diffusion model.Proceedings. Biological sciences · 2024Article
- Germline Cas9 promoters with improved performance for homing gene drive.Nature communications · 2024Article
- Article
- A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.Nature communications · 2024Article
- A Photoresponsive Homing Endonuclease for Programmed DNA Cleavage.ACS synthetic biology · 2024Article
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- Closing the gap to effective gene drive in Aedes aegypti by exploiting germline regulatory elements.Nature communications · 2023Article
- Assessment of distant-site rescue elements for CRISPR toxin-antidote gene drives.Frontiers in bioengineering and biotechnology · 2023Article
- Dichotomous sperm in Lepidopteran insects: a biorational target for pest management.Frontiers in insect science · 2023Review
- A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias.Nature communications · 2022Article
- Daisy-chain gene drives: The role of low cut-rate, resistance mutations, and maternal deposition.PLoS genetics · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Making discrete and precise genetic changes to wild populations has been proposed as a means of addressing some of the world's most pressing ecological and public health challenges caused by insect pests. Technologies that would allow this, such as synthetic gene drives, have been under development for many decades. Recently, a new generation of programmable nucleases has dramatically accelerated technological development. CRISPR-Cas9 has improved the efficiency of genetic engineering and has been used as the principal effector nuclease in different gene drive inheritance biasing mechanisms. Of these nuclease-based gene drives, homing endonuclease gene drives have been the subject of the bulk of research efforts (particularly in insects), with many different iterations having been developed upon similar core designs. We chart the history of homing gene drive development, highlighting the emergence of challenges such as unintended repair outcomes, "leaky" expression, and parental deposition. We conclude by discussing the progress made in developing strategies to increase the efficiency of homing endonuclease gene drives and mitigate or prevent unintended outcomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.