ArticleCell reports2022
Yeast cell death pathway requiring AP-3 vesicle trafficking leads to vacuole/lysosome membrane permeabilization.
Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Crystal Facet-Polysaccharide Matching in CFS-P Nanocrystals Drives Fungal Uptake, Vacuole Destruction, and Selective Antifungal Activity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The heat-ramp method to study regulated cell death in a pathogenic yeastbioRxiv : the preprint server for biology · 2026Article
- Using CMAC Staining for Vacuole Characterization in Yeast.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A method for authenticating the fidelity ofMicrobiology spectrum · 2025Article
- Cell differentiation, aging, and death in spatially organized yeast communities: mechanisms and consequences.Cell death and differentiation · 2025Review
- Peroxisomes regulate virulence and cell density sensing inbioRxiv : the preprint server for biology · 2025Article
- Vacuolar sterol β-glucosidase EGCrP2/Sgl1 deficiency in Cryptococcus neoformans: Dysfunctional autophagy and Mincle-dependent immune activation as targets of novel antifungal strategies.PLoS pathogens · 2025Article
- Current and novel approaches in yeast cell death research.Cell death and differentiation · 2025Review
- Exploring Sugar-Induced Cell Death (SICD) in Yeast: Implications for Diabetes and Cancer Research.Frontiers in cell death · 2025Article
- Environmental fungi from cool and warm neighborhoods in the heat island of Baltimore City show differences in thermal susceptibility and pigmentation.ISME communications · 2025Article
- Exploring Saccharomycotina Yeast Ecology Through an Ecological Ontology Framework.Yeast (Chichester, England) · 2024Review
- Identification of programmed cell death-related genes and diagnostic biomarkers in endometriosis using a machine learning and Mendelian randomization approach.Frontiers in endocrinology · 2024Article
- Article
- Programmed Cell Death in Unicellular Versus Multicellular Organisms.Annual review of genetics · 2023Review
- A dynamic actin cytoskeleton is required to prevent constitutive VDAC-dependent MAPK signalling and aberrant lipid homeostasis.iScience · 2023Article
- Similar evolutionary trajectories in an environmentalProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The emerging role of extracellular vesicles in fungi: a double-edged sword.Frontiers in microbiology · 2023Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Unicellular eukaryotes have been suggested as undergoing self-inflicted destruction. However, molecular details are sparse compared with the mechanisms of programmed/regulated cell death known for human cells and animal models. Here, we report a molecular cell death pathway in Saccharomyces cerevisiae leading to vacuole/lysosome membrane permeabilization. Following a transient cell death stimulus, yeast cells die slowly over several hours, consistent with an ongoing molecular dying process. A genome-wide screen for death-promoting factors identified all subunits of the AP-3 complex, a vesicle trafficking adapter known to transport and install newly synthesized proteins on the vacuole/lysosome membrane. To promote cell death, AP-3 requires its Arf1-GTPase-dependent vesicle trafficking function and the kinase Yck3, which is selectively transported to the vacuole membrane by AP-3. Video microscopy revealed a sequence of events where vacuole permeability precedes the loss of plasma membrane integrity. AP-3-dependent death appears to be conserved in the human pathogenic yeast Cryptococcus neoformans.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.