ArticleCell reports2022
The Arabidopsis F-box protein FBW2 targets AGO1 for degradation to prevent spurious loading of illegitimate small RNA.
Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Diverse RNA viral effectors converge on facilitation of AGO4 degradation to promote infection.Nucleic acids research · 2025Article
- Oxidative stress: a new player in the regulation of AGO1 homeostasis?Journal of experimental botany · 2025Article
- Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner.Journal of experimental botany · 2025Article
- Post-Translational Modifications of TOE3 Regulate Antiviral Defense in Tobacco.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The nuclear pore complex acts as a hub for pri-miRNA transcription and processing in plants.Nucleic acids research · 2025Article
- Remote precursor elements can modulate RNA induced silencing complex-loading efficiency of miR168 in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- Importance of an N-terminal structural switch in the distinction between small RNA-bound and free ARGONAUTE.Nature structural & molecular biology · 2025Article
- HC-Pro inhibits HEN1 methyltransferase activity, leading to autophagic degradation of AGO1.Nature communications · 2025Article
- The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Transcriptome-wide Identification of Nine Tandem Repeat Protein Families in Roselle (Tropical life sciences research · 2024Article
- Proteolytic control of the RNA silencing machinery.The Plant cell · 2024Review
- Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications.Nucleic acids research · 2024Article
- The unique dual targeting of AGO1 by two types of PRMT enzymes promotes phasiRNA loading in Arabidopsis thaliana.Nucleic acids research · 2024Article
- Heat stress promotes Arabidopsis AGO1 phase separation and association with stress granule components.iScience · 2024Article
- The plant siRNA landscape.The Plant cell · 2024Review
- Identification and Functional Analysis of the Promoter of a Leucoanthocyanidin Reductase Gene from Gossypium hirsutum.Molecular biotechnology · 2023Article
- Promiscuous splicing-derived hairpins are dominant substrates of tailing-mediated defense of miRNA biogenesis in mammals.Cell reports · 2023Article
- Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Pro.Virology journal · 2023Article
- microRNA production inFrontiers in plant science · 2023Review
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing is a conserved mechanism in eukaryotes involved in development and defense against viruses. In plants, ARGONAUTE1 (AGO1) protein plays a central role in both microRNA- and small interfering RNA-directed silencing, and its expression is regulated at multiple levels. Here, we report that the F-box protein FBW2 assembles an SCF complex that selectively targets for proteolysis AGO1 when it is unloaded and mutated. Although FBW2 loss of function does not lead to strong growth or developmental defects, it significantly increases RNA-silencing activity. Interestingly, under conditions in which small-RNA accumulation is affected, the failure to degrade AGO1 in fbw2 mutants becomes more deleterious for the plant. Accordingly, the non-degradable AGO1 protein assembles high-molecular-weight complexes and binds illegitimate small RNA, leading to off-target cleavage. Therefore, control of AGO1 homeostasis by FBW2 plays an important role in quality control of RNA silencing.
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