Evidence map›Paper›PMID 35415968›Full record

ArticleAnimal models and experimental medicine2022

A 3D-printable device allowing fast and reproducible longitudinal preparation of mouse intestines.

Beckey DeLucia, Sergey Samorezov, Megan T Zangara, Rachel L Markley, Lucas J Osborn, Karlee B Schultz, Christine McDonald, Jan Claesen

Abstract read
In one paragraph

Article in Animal models and experimental medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Functional Diversity of Memory CD8 T Cells is Spatiotemporally Imprinted.bioRxiv : the preprint server for biology · 2024
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Beckey DeLuciaDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.ORCID 0000-0001-5895-2408
Sergey SamorezovDepartment of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.
Megan T ZangaraDepartment of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio, USA.ORCID 0000-0002-0884-9936
Rachel L MarkleyDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.ORCID 0000-0003-2114-708X
Lucas J OsbornDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.ORCID 0000-0003-0077-9192
Karlee B SchultzDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.ORCID 0000-0001-8661-9784
Christine McDonaldDepartment of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio, USA.ORCID 0000-0002-6745-9487
Jan ClaesenDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.ORCID 0000-0002-0755-7974

Funding

TUMOR METABOLISM PROGRAMP30CA043703 · NCI · CASE WESTERN RESERVE UNIVERSITY · PI Amar Desai · 1987 to 2026
$142.3M
Uncovering aryl polyene biology to identify new drug targets in Gram-negative bacterial pathogensR01AI153173 · NIAID · CLEVELAND CLINIC LERNER COM-CWRU · PI CLAESEN, JAN · 2021 to 2025
$2.0M
NCI NIH HHS P30 CA043703NIAID NIH HHS R01 AI153173
6 · The paper itself

Abstract

Accurate and reproducible analysis of murine small and large intestinal tissue is key for preclinical models involving intestinal pathology. Currently, there is no easily accessible, standardized method that allows researchers of different skill levels to consistently dissect intestines in a time-efficient manner. Here, we describe the design and use of the 3D-printed "Mouse Intestinal Slicing Tool" (MIST), which can be used to longitudinally dissect murine intestines for further analysis. We benchmarked the MIST against a commonly used procedure involving scissors to make a longitudinal cut along the intestines. Use of the MIST halved the time per mouse to prepare the intestines and outperformed alternative methods in smoothness of the cutting edge and overall reproducibility. By sharing the plans for printing the MIST, we hope to contribute a uniformly applicable method for saving time and increasing consistency in studies of the mouse gastrointestinal tract.

Indexed as

IntestinesPrinting, Three-DimensionalAnimalsMiceReproducibility of Resultsadenomadissectionintestinesmiceprinting, three-dimensionalreproducibility of results

Identifiers

PMID35415968
PMCPMC9043725

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.