ArticleFood chemistry. Molecular sciences2022
Detection and quantification of adulteration in milk and dairy products: A novel and sensitive qPCR-based method.
Article in Food chemistry. Molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 44 citations in OpenAlex.
- Development and evaluation of a real-time PCR assay for the detection of bovine Milk in foods.Food chemistry. Molecular sciences · 2026Article
- An ARMS-integrated high-resolution melting assay for reliable discrimination of the A1/A2 polymorphism in the bovineFood chemistry. Molecular sciences · 2026Article
- Simultaneous Detection of Eight Dairy-Derived Components Using Double-Tube Multiplex qPCR Based TaqMan Probe.Foods (Basel, Switzerland) · 2024Article
- Analysis of species adulteration in beef sausage using real-time polymerase chain reaction in Makassar, Indonesia.Veterinary world · 2024Article
- Photonic Dipstick Immunosensor to Detect Adulteration of Ewe, Goat, and Donkey Milk with Cow Milk through Bovine κ-Casein Detection.Sensors (Basel, Switzerland) · 2024Article
- A multiplex DNA probe-based method for simultaneous identification of adulteration in meat samples.Food chemistry. Molecular sciences · 2024Article
- Novel and Sensitive Touchdown Polymerase Chain Reaction Assays for the Detection of Goat and Sheep Milk Adulteration with Cow Milk.Molecules (Basel, Switzerland) · 2024Article
- A droplet digital PCR method for the detection of scale drop disease virus in yellowfin seabream (Frontiers in microbiology · 2024Article
- Detection of Soybean-Derived Components in Dairy Products Using Proofreading Enzyme-Mediated Probe Cleavage Coupled with Ladder-Shape Melting Temperature Isothermal Amplification (Proofman-LMTIA).Molecules (Basel, Switzerland) · 2023Article
- How Chemometrics Can Fight Milk Adulteration.Foods (Basel, Switzerland) · 2022Review
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5 authors at 1 institution in 1 country.
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Abstract
Species identification in dairy products has a notable importance in food traceability and adulteration control and consequently has a significant effect on the final economic value of foods. In the present study, we developed a method based on real-time quantitative PCR (qPCR) for detection and quantification of cow DNA in DNA samples from milk and dairy products from buffaloes, goats, and sheep. The qPCR reactions showed high specificity, and the amplifications only occurred to species-specific primers. The calibration curves allowed for the quantification of the amount of DNA of each species-specific primer, and the established detection limit was 0.016 ng for the four species. The detection limit of cow DNA in buffalo, goat and sheep DNA samples was 0.1% (0.01 ng). Although the present study aimed to detect and quantify cow DNA in buffalo, goat, and sheep dairy products, we believe that the qPCR assays can also be directed to differentiate and quantify goat × sheep, and/or buffalo × goat/sheep.
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