ArticleBiomedical optics express2022
Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy.
Article in Biomedical optics express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Two-photon light-sheet microscopy with isotropic near-diffraction-limited resolution in three dimensions.iScience · 2026Article
- Fast volumetric imaging of a zebrafish seizure model with adaptive optics light sheet microscopy.Biomedical optics express · 2026Article
- Employing zebrafish to understand genetic drivers of epilepsy-related comorbid behaviors.Frontiers in pharmacology · 2026Article
- Zebrafish as a translational model for peptide-mediated synaptic dysfunction related to obesity.Frontiers in synaptic neuroscience · 2026Review
- In vivo three-photon fluorescence imaging of mouse brain vasculature labeled by Evans blue excited at the NIR-III window.Biomedical optics express · 2025Article
- Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity.Communications biology · 2024Article
- Neural Basis of Number Sense in Larval Zebrafish.bioRxiv : the preprint server for biology · 2024Article
- Chronotropic and vasoactive properties of the gut bacterial short-chain fatty acids in larval zebrafish.Physiological genomics · 2024Article
- Introduction to the Optics and the Brain 2023 feature issue.Biomedical optics express · 2024Article
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Article
- Epilepsy insights revealed by intravital functional optical imaging.Frontiers in neurology · 2024Review
- Imaging Approaches to Investigate Pathophysiological Mechanisms of Brain Disease in Zebrafish.International journal of molecular sciences · 2023Review
- Enhanced neuroimaging with a calcium sensor inJournal of biomedical optics · 2023Article
- Zebrafish as an Innovative Tool for Epilepsy Modeling: State of the Art and Potential Future Directions.International journal of molecular sciences · 2023Review
- Small fish, big discoveries: zebrafish shed light on microbial biomarkers for neuro-immune-cardiovascular health.Frontiers in physiology · 2023Review
- Structural and functional imaging of brains.Science China. Chemistry · 2023Review
- Extended depth-of-field light-sheet microscopy improves imaging of large volumes at high numerical aperture.Applied physics letters · 2022Article
- Multimodal Characterization of Seizures in Zebrafish Larvae.Biomedicines · 2022Article
- Quantity as a Fish Views It: Behavior and Neurobiology.Frontiers in neuroanatomy · 2022Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one-photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The use of two-photon (2P) excitation, employing near-infrared invisible light, provides unbiased investigation of neuronal circuit dynamics. However, due to the low efficiency of the 2P absorption process, the imaging speed of this technique is typically limited by the signal-to-noise-ratio. Here, we describe a 2P LSFM setup designed for non-invasive imaging that enables quintuplicating state-of-the-art volumetric acquisition rate of the larval zebrafish brain (5 Hz) while keeping low the laser intensity on the specimen. We applied our system to the study of pharmacologically-induced acute seizures, characterizing the spatial-temporal dynamics of pathological activity and describing for the first time the appearance of caudo-rostral ictal waves (CRIWs).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.