ArticleGenome biology2022
Strand asymmetry influences mismatch resolution during a single-strand annealing.
Article in Genome biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Machine learning prediction of prime editing efficiency across diverse chromatin contexts.Nature biotechnology · 2025Article
- Nonhomologous tails direct heteroduplex rejection and mismatch correction during single-strand annealing in Saccharomyces cerevisiae.PLoS genetics · 2024Article
- Strand asymmetry influences mismatch resolution during a single-strand annealing.Genome biology · 2022Article
- Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance.Molecular cell · 2021Article
- Protocol: A Multiplexed Reporter Assay to Study Effects of Chromatin Context on DNA Double-Strand Break Repair.Frontiers in genetics · 2021Article
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiases of DNA repair can shape the nucleotide landscape of genomes at evolutionary timescales. The molecular mechanisms of those biases are still poorly understood because it is difficult to isolate the contributions of DNA repair from those of DNA damage.
resultsHere, we develop a genome-wide assay whereby the same DNA lesion is repaired in different genomic contexts. We insert thousands of barcoded transposons carrying a reporter of DNA mismatch repair in the genome of mouse embryonic stem cells. Upon inducing a double-strand break between tandem repeats, a mismatch is generated if the break is repaired through single-strand annealing. The resolution of the mismatch showed a 60-80% bias in favor of the strand with the longest 3' flap. The location of the lesion in the genome and the type of mismatch had little influence on the bias. Instead, we observe a complete reversal of the bias when the longest 3' flap is moved to the opposite strand by changing the position of the double-strand break in the reporter.
conclusionsThese results suggest that the processing of the double-strand break has a major influence on the repair of mismatches during a single-strand annealing.
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