Evidence map›Paper›PMID 35413133›Full record

ArticleThe Journal of physiology2022

Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters.

Francesca Vacca, Ana S Gomes, Koji Murashita, Raffella Cinquetti, Cristina Roseti, Amilcare Barca, Ivar Rønnestad, Tiziano Verri, Elena Bossi

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
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  5. Cell-based homologous expression system forCurrent research in physiology · 2024
    Article
  6. The "www" ofMembranes · 2022
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Francesca VaccaLaboratory of Cellular and Molecular Physiology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Ana S GomesDepartment of Biological Sciences, University of Bergen, Bergen, Norway.ORCID 0000-0002-2520-7070
Koji MurashitaResearch Center for Aquaculture Systems, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Minami-ise, Mie, Japan.
Raffella CinquettiLaboratory of Cellular and Molecular Physiology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Cristina RosetiLaboratory of Cellular and Molecular Physiology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Amilcare BarcaLaboratory of Applied Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.ORCID 0000-0002-3117-2724
Ivar RønnestadDepartment of Biological Sciences, University of Bergen, Bergen, Norway.ORCID 0000-0001-8789-0197
Tiziano VerriLaboratory of Applied Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.ORCID 0000-0003-4983-2767
Elena BossiLaboratory of Cellular and Molecular Physiology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.ORCID 0000-0002-9549-2153
University of Insubria · ITUniversity of Bergen · NOUniversity of Salento · ITJapan Fisheries Research and Education Agency · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The high-affinity/low-capacity system Slc15a2 (PepT2) is responsible for the reuptake of di/tripeptides from the renal proximal tubule, but it also operates in many other tissues and organs. Information regarding PepT2 in teleost fish is limited and, to date, functional data are available from the zebrafish (Danio rerio) only. Here, we report the identification of two slc15a2 genes in the Atlantic salmon (Salmo salar) genome, namely slc15a2a and slc15a2b. The two encoded PepT2 proteins share 87% identity and resemble both structurally and functionally the canonical vertebrate PepT2 system. The mRNA tissue distribution analyses reveal a widespread distribution of slc15a2a transcripts, being more abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and the distal part of the gastrointestinal tract. The function of the two transporters was investigated by heterologous expression in Xenopus laevis oocytes and two-electrode voltage-clamp recordings of transport and presteady-state currents. Both PepT2a and PepT2b in the presence of Gly-Gln elicit pH-dependent and Na

Indexed as

Salmo salarSymportersAnimalsKineticsMammalsOocytesRatsZebrafishSymportersDi/tripeptide transport(ers)PepT2aPepT2bpresteady-state currentsSlc15a2aSlc15a2btwo-electrode voltage-clampwhole-genome duplicationXenopus laevis oocytes

Identifiers

PMID35413133
PMCPMC9321897
OpenAlexW4226060352

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.