Evidence map›Paper›PMID 35412917›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2022

A high-affinity cocaine binding site associated with the brain acid soluble protein 1.

Maged M Harraz, Adarsha P Malla, Evan R Semenza, Maria Shishikura, Manisha Singh, Yun Hwang, In Guk Kang, Young Jun Song, Adele M Snowman, Pedro Cortés and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 1 institution in 1 country.

Maged M HarrazThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Adarsha P MallaThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Evan R SemenzaThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Maria ShishikuraThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Manisha SinghThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Yun HwangThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
In Guk KangThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Young Jun SongThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Adele M SnowmanThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Pedro CortésThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Senthilkumar S KaruppagounderNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Ted M DawsonThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Valina L DawsonThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.ORCID 0000-0002-2915-3970
Solomon H SnyderThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.ORCID 0000-0002-6818-7022
Johns Hopkins University · US

Funding

UNDERSTANDING NO SIGNALING RESULTING IN NEUROPROTECTION.P50DA000266 · NIDA · JOHNS HOPKINS UNIVERSITY · PI BARABAN, JAY M · 1985 to 2017
$45.2M
Understanding Mechanisms of a-synuclein pathologyP50NS038377 · NINDS · JOHNS HOPKINS UNIVERSITY · PI DAWSON, TED M. · 1998 to 2019
$38.8M
Thorase Regulation of the Actions of CocaineP50DA044123 · NIDA · JOHNS HOPKINS UNIVERSITY · PI DAWSON, VALINA L., JANAK, PATRICIA H. · 2018 to 2022
$9.0M
HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS) NS38377HHS | NIH | National Institute on Drug Abuse (NIDA) DA00266HHS | NIH | National Institute on Drug Abuse (NIDA) DA044123NIDA NIH HHS P50 DA000266NIDA NIH HHS P50 DA044123NINDS NIH HHS P50 NS038377
6 · The paper itself

Abstract

Cocaine exerts its stimulant effect by inhibiting dopamine (DA) reuptake, leading to increased dopamine signaling. This action is thought to reflect the binding of cocaine to the dopamine transporter (DAT) to inhibit its function. However, cocaine is a relatively weak inhibitor of DAT, and many DAT inhibitors do not share cocaine’s behavioral actions. Further, recent reports show more potent actions of the drug, implying the existence of a high-affinity receptor for cocaine. We now report high-affinity binding of cocaine associated with the brain acid soluble protein 1 (BASP1) with a dissociation constant (Kd) of 7 nM. Knocking down BASP1 in the striatum inhibits [3H]cocaine binding to striatal synaptosomes. Depleting BASP1 in the nucleus accumbens but not the dorsal striatum diminishes locomotor stimulation in mice. Our findings imply that BASP1 is a pharmacologically relevant receptor for cocaine.

Indexed as

Calmodulin-Binding ProteinsCarrier ProteinsCocaineCytoskeletal ProteinsNerve Tissue ProteinsReceptors, DrugAnimalsBinding SitesCorpus StriatumDopamineDopamine Plasma Membrane Transport ProteinsGene Knock-In TechniquesHumansMiceRatsBasp1 protein, mouseCalmodulin-Binding ProteinsCarrier ProteinsCocainecocaine receptorCytoskeletal ProteinsDopamineDopamine Plasma Membrane Transport ProteinsNerve Tissue ProteinsReceptors, DrugBASP1behaviorcocainedrug abusereceptor

Identifiers

PMID35412917
PMCPMC9169839
OpenAlexW4223570065

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.