ArticleProceedings of the National Academy of Sciences of the United States of America2022
Engineered plant control of associative nitrogen fixation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 69 citations in OpenAlex.
- Challenges and Future Directives of Synthetic Biology in Engineering Plant-Microbe Partnerships for Sustainable Agriculture.Biotechnology and bioengineering · 2026Review
- Beyond alienness: An impact-centred framework for plant invasions.Plant diversity · 2026Article
- Quantitative Measurement of Synthetic Repression Curves Reveals Design Challenges for Genetic Circuit Engineering under Growth Arrest.ACS synthetic biology · 2026Article
- The metabolic and anatomical complexity of root microhabitats modulate their interaction with the microbiota.Nature communications · 2026Article
- Engineered diazotrophs with host-inducible nitrogen supply systems: Transforming rice farming through innovative nitrogen biofertilizers.Journal of integrative plant biology · 2026Review
- Bag-controlled low-nitrogen fertilization induces root arbutin secretion to enrich rhizosphere Pseudomonas and enhance nitrogen fixation in peach.Plant communications · 2026Article
- mGem: Applying microbiome therapeutic learnings to next-generation agricultural bioproducts.mBio · 2026Review
- Nitrogen-Controlled Host Gatekeeping: Regulatory Chokepoints Across Four Windows for Diazotroph Access.International journal of molecular sciences · 2026Article
- CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization.Nature communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Increased Apigenin in DNA-Edited Hexaploid Wheat Promoted Soil Bacterial Nitrogen Fixation and Improved Grain Yield Under Limiting Nitrogen Fertiliser.Plant biotechnology journal · 2025Article
- Harnessing plant agriculture to mitigate climate change: A framework to evaluate synthetic biology (and other) interventions.Plant physiology · 2025Article
- Nitrogen Use Efficiency in Agriculture: Integrating Biotechnology, Microbiology, and Novel Delivery Systems for Sustainable Agriculture.Plants (Basel, Switzerland) · 2025Review
- Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility.Plant cell reports · 2025Article
- Utilizing plant synthetic biology to accelerate plant-microbe interactions research.Biodesign research · 2025Review
- Design and regulation of engineered bacteria for environmental release.Nature microbiology · 2025Review
- Phenylpropanoid methyl esterase unlocks catabolism of aromatic biological nitrification inhibitors.The ISME journal · 2025Article
- Optimizing nitrogen use efficiency in forest plantations: mechanistic insights fromForestry research · 2025Review
- Regulation of Rhizobial Nodulation Genes by Flavonoid-Independent NodD Supports Nitrogen-Fixing Symbioses With Legumes.Environmental microbiology · 2025Article
- Biotechnological advances in plant growth-promoting rhizobacteria for sustainable agriculture.World journal of microbiology & biotechnology · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
Engineering N2-fixing symbioses between cereals and diazotrophic bacteria represents a promising strategy to sustainably deliver biologically fixed nitrogen (N) in agriculture. We previously developed novel transkingdom signaling between plants and bacteria, through plant production of the bacterial signal rhizopine, allowing control of bacterial gene expression in association with the plant. Here, we have developed both a homozygous rhizopine producing (RhiP) barley line and a hybrid rhizopine uptake system that conveys upon our model bacterium Azorhizobium caulinodans ORS571 (Ac) 103-fold improved sensitivity for rhizopine perception. Using this improved genetic circuitry, we established tight rhizopine-dependent transcriptional control of the nitrogenase master regulator nifA and the N metabolism σ-factor rpoN, which drove nitrogenase expression and activity in vitro and in situ by bacteria colonizing RhiP barley roots. Although in situ nitrogenase activity was suboptimally effective relative to the wild-type strain, activation was specific to RhiP barley and was not observed on the roots of wild-type plants. This work represents a key milestone toward the development of a synthetic plant-controlled symbiosis in which the bacteria fix N2 only when in contact with the desired host plant and are prevented from interaction with nontarget plant species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.