ArticleThe Journal of antimicrobial chemotherapy2022
Population structure of blaKPC-harbouring IncN plasmids at a New York City medical centre and evidence for multi-species horizontal transmission.
Article in The Journal of antimicrobial chemotherapy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Molecular Insights into Carbapenem Resistance inInternational journal of molecular sciences · 2026Review
- Genomic convergence of mobilome-associated multidrug resistance and host-adaptation determinants in a postoperativeFrontiers in cellular and infection microbiology · 2026Article
- Comparative metagenomics using pan-metagenomic graphs.bioRxiv : the preprint server for biology · 2025Article
- Global epidemiological insights on the antimicrobial resistance ofMicrobial genomics · 2025Article
- Plasmid conjugation drives within-patient plasmid diversity.Microbial genomics · 2025Article
- Article
- Genomic investigation of multispecies and multivariantInfection control and hospital epidemiology · 2024Article
- Molecular Characteristics and Antimicrobial Susceptibility Profiles ofInfection and drug resistance · 2024Article
- First Detection of International High-RiskTransboundary and emerging diseases · 2024Article
- Characterization ofAntibiotics (Basel, Switzerland) · 2023Article
- Detection of IMP-4 and SFO-1 co-producing ST51Frontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundCarbapenem-resistant Enterobacterales (CRE) are highly concerning MDR pathogens. Horizontal transfer of broad-host-range IncN plasmids may contribute to the dissemination of the Klebsiella pneumoniae carbapenemase (KPC), spreading carbapenem resistance among unrelated bacteria. However, the population structure and genetic diversity of IncN plasmids has not been fully elucidated.
objectivesWe reconstructed blaKPC-harbouring IncN plasmid genomes to characterize shared gene content, structural variability, and putative horizontal transfer within and across patients and diverse bacterial clones.
methodsWe performed short- and long-read sequencing and hybrid assembly on 45 CRE isolates with blaKPC-harbouring IncN plasmids. Eight serial isolates from two patients were included to assess intra-patient plasmid dynamics. Comparative genomic analysis was performed to assess structural and sequence similarity across plasmids. Within IncN sublineages defined by plasmid MLST and kmer-based clustering, phylogenetic analysis was used to identify closely related plasmids.
resultsComparative analysis of IncN plasmid genomes revealed substantial heterogeneity including large rearrangements in serial patient plasmids and differences in structure and content across plasmid clusters. Within plasmid sublineages, core genome content and resistance gene regions were largely conserved. Closely related plasmids (≤1 SNP) were found in highly diverse isolates, including ten pST6 plasmids found in eight bacterial clones from three different species.
conclusionsGenomic analysis of blaKPC-harbouring IncN plasmids revealed the presence of several distinct sublineages as well as substantial host diversity within plasmid clusters suggestive of frequent mobilization. This study reveals complex plasmid dynamics within a single plasmid family, highlighting the challenge of tracking plasmid-mediated transmission of blaKPC in clinical settings.
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