Evidence map›Paper›PMID 35412277›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses.

Wesley M Tierney, Ian A Vicino, Stella Y Sun, Wah Chiu, Esteban A Engel, Matthew P Taylor, Ian B Hogue

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. LIVE-CELL FLUORESCENCE MICROSCOPY OF HSV-1 CELLULAR EGRESS BY EXOCYTOSIS.bioRxiv : the preprint server for biology · 2023
    Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wesley M Tierney *Center for Immunotherapy, Vaccines, and Virotherapy, Biodesign Institute, and School of Life Sciences, Arizona State University, Tempe, AZ, USA.
Ian A Vicino *Center for Immunotherapy, Vaccines, and Virotherapy, Biodesign Institute, and School of Life Sciences, Arizona State University, Tempe, AZ, USA.
Stella Y SunDepartment of Bioengineering, Department of Microbiology and Immunology, Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA, USA.
Wah ChiuDepartment of Bioengineering, Department of Microbiology and Immunology, Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA, USA.
Esteban A EngelDepartment of Molecular Biology and Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA.
Matthew P TaylorDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA. mptaylor@montana.edu.
Ian B HogueCenter for Immunotherapy, Vaccines, and Virotherapy, Biodesign Institute, and School of Life Sciences, Arizona State University, Tempe, AZ, USA. ihogue@asu.edu.

Funding

3 D ELECTRON MICROSCOPY OF MACROMOLECULES (5P41RR002250-27)P41GM103832 · NIGMS · STANFORD UNIVERSITY · PI CHIU, WAH · 2012 to 2019
$15.1M
ResourceP40OD010996 · OD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI STRICK, PETER · 2012 to 2024
$11.0M
Identifying heterogenous neuronal responses to HSV-1 infection with drop-based microfluidicsR21AI171724 · NIAID · MONTANA STATE UNIVERSITY - BOZEMAN · PI TAYLOR, MATTHEW P. · 2023 to 2024
$399k
Evaluation of innate antiviral responses on neuronal spread of HSV-1 infectionR21AI146952 · NIAID · MONTANA STATE UNIVERSITY - BOZEMAN · PI TAYLOR, MATTHEW P. · 2020 to 2021
$396k
Molecular and Cellular Mechanisms of Herpes Simplex Transport and Egress Pathway in NeuronsK22AI123159 · NIAID · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI HOGUE, IAN B · 2017 to 2018
$261k
NIAID NIH HHS K22 AI123159NIAID NIH HHS R21 AI146952NIAID NIH HHS R21 AI171724NIGMS NIH HHS P41 GM103832NIH HHS P40 OD010996
6 · The paper itself

Abstract

The development of compartmentalized neuron culture systems has been invaluable in the study of neuroinvasive viruses, including the alpha herpesviruses Herpes Simplex Virus 1 (HSV-1) and Pseudorabies Virus (PRV). This chapter provides updated protocols for assembling and culturing rodent embryonic superior cervical ganglion (SCG) and dorsal root ganglion (DRG) neurons in Campenot trichamber cultures. In addition, we provide several illustrative examples of the types of experiments that are enabled by Campenot cultures: (1) Using fluorescence microscopy to investigate axonal outgrowth/extension through the chambers, and alpha herpesvirus infection, intracellular trafficking, and cell-cell spread via axons. (2) Using correlative fluorescence microscopy and cryo electron tomography to investigate the ultrastructure of virus particles trafficking in axons.

Indexed as

Herpesvirus 1, HumanHerpesvirus 1, SuidAnimalsAxonal TransportAxonsNeuronsAlphaherpesvirusCampenotCryo electron tomographyDorsal root gangliaFluorescence microscopyHerpes simplex virusNeuronsPseudorabies virusSuperior cervical gangliaVirus

Identifiers

PMID35412277
PMCPMC10427112

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.