Evidence map›Paper›PMID 35412256›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes.

Boris Fichtman, Saroj G Regmi, Mary Dasso, Amnon Harel

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Boris FichtmanAzrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel.
Saroj G RegmiDivision of Molecular and Cellular Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Mary DassoDivision of Molecular and Cellular Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Amnon HarelAzrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel. amnon.harel@biu.ac.il.
Bar-Ilan University · ILEunice Kennedy Shriver National Institute of Child Health and Human Development · US

Funding

Mitotic roles of the Nuclear Transport MachineryZIAHD008954 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI DASSO, MARY C. · 2015 to 2025
$25.3M
Intramural NIH HHS ZIA HD008954
6 · The paper itself

Abstract

Field emission scanning electron microscopy (FESEM) is a well-established technique for acquiring three-dimensional surface images of nuclear pore complexes (NPCs). We present an optimized protocol for the exposure of mammalian cell nuclei and direct surface imaging of nuclear envelopes by FESEM, allowing for a detailed morphological comparison of individual NPCs, without the need for averaging techniques. This provides a unique high resolution tool for studying the effects of cellular stress, specific genetic manipulations and inherited diseases on the ultrastructure of human NPCs.

Indexed as

Nuclear EnvelopeNuclear PoreAnimalsCell NucleusHumansImaging, Three-DimensionalMammalsMicroscopy, Electron, ScanningAuxin-inducible degronCentral channel particlesMammalian cell cultureNuclear envelopeNuclear pore complexScanning electron microscopy

Identifiers

PMID35412256
PMCPMC11166266
OpenAlexW4225892130

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.