Evidence map›Paper›PMID 35412252›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts.

Sampada Mishra, Daniel L Levy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Reconstitution of genome remodeling usingFrontiers in cell and developmental biology · 2026
    Review
  2. How the chromatin landscape influences nuclear morphology.Frontiers in cell and developmental biology · 2025
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Sampada MishraDepartment of Molecular Biology, University of Wyoming, Laramie, WY, USA.
Daniel L LevyDepartment of Molecular Biology, University of Wyoming, Laramie, WY, USA. dlevy1@uwyo.edu.

Funding

Wyoming INBRE Phase 4- Equipment Supplement for x-ray diffractometer for Center for Advanced Scientific InstrumentationP20GM103432 · NIGMS · UNIVERSITY OF WYOMING · PI Robert Scott Seville · 2012 to 2026
$56.8M
Mechanisms of nuclear size regulationR35GM134885 · NIGMS · UNIVERSITY OF WYOMING · PI Daniel Leon Levy · 2020 to 2026
$2.5M
NIGMS NIH HHS P20 GM103432NIGMS NIH HHS R35 GM134885
6 · The paper itself

Abstract

The nuclear pore complex (NPC) is the conduit in the nuclear envelope through which proteins and RNA are transported between the cytoplasm and nucleus. Xenopus egg extracts that support de novo assembly of nuclei have provided a robust system to study NPC structure and function because the biochemical composition of the extract can be easily manipulated. Here we describe how to assemble nuclei in Xenopus egg extract, how to visualize and analyze NPCs in both live and fixed samples, and different approaches to altering nucleocytoplasmic transport in extract.

Indexed as

Nuclear EnvelopeNuclear PoreNuclear Pore Complex ProteinsActive Transport, Cell NucleusAnimalsCell NucleusMicroscopyXenopus laevisNuclear Pore Complex ProteinsImmunofluorescencemAb414NPCNuclear importNuclear pore complexesSpindownXenopus egg extracts

Identifiers

PMID35412252
PMCPMC9219414

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.