Evidence map›Paper›PMID 35412250›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Speed Microscopy: High-Speed Single Molecule Tracking and Mapping of Nucleocytoplasmic Transport.

Steven J Schnell, Mark Tingey, Weidong Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Steven J SchnellDepartment of Biology, Temple University, Philadelphia, PA, USA.
Mark TingeyDepartment of Biology, Temple University, Philadelphia, PA, USA.
Weidong YangDepartment of Biology, Temple University, Philadelphia, PA, USA. weidong.yang@temple.edu.

Funding

Super-resolution Microscopy Study of Molecular Transport MechanismsR35GM122552 · NIGMS · TEMPLE UNIV OF THE COMMONWEALTH · PI Weidong Yang · 2017 to 2026
$4.5M
Structure and Function of the Ciliary Pore ComplexR01GM116204 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI VERHEY, KRISTEN J., YANG, WEIDONG · 2015 to 2018
$2.1M
Three-Dimensional Super-Resolution Microscopy Study of Nuclear TransportR01GM097037 · NIGMS · TEMPLE UNIV OF THE COMMONWEALTH · PI YANG, WEIDONG · 2011 to 2015
$1.3M
NIGMS NIH HHS R01 GM097037NIGMS NIH HHS R01 GM116204NIGMS NIH HHS R35 GM122552
6 · The paper itself

Abstract

The nuclear pore complex (NPC) functions as a gateway through which molecules translocate into and out of the nucleus. Understanding the transport dynamics of these transiting molecules and how they interact with the NPC has great potentials in the discovery of clinical targets. Single-molecule microscopy techniques are powerful tools to provide sub-diffraction limit information about the dynamic and structural details of nucleocytoplasmic transport. Here we detail single-point edge-excitation subdiffraction (SPEED) microscopy, a high-speed superresolution microscopy technique designed to track and map proteins and RNAs as they cross native NPCs.

Indexed as

Nuclear PoreSingle Molecule ImagingActive Transport, Cell NucleusMicroscopyNuclear Pore Complex ProteinsNuclear Pore Complex ProteinsLive cell imagingNucleocytoplasmic transportSingle-molecule microscopySingle-point edge-excitation subdiffraction microscopySub–diffraction limit imagingSuperresolution light microscopy

Identifiers

PMID35412250
PMCPMC10131132

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.