Evidence map›Paper›PMID 35411481›Full record

ArticleMolecular biology reports2022

Novel piRNA MW557525 regulates the growth of Piwil2-iCSCs and maintains their stem cell pluripotency.

Liming Jin, Zhaoxia Zhang, Zhang Wang, Xiaojun Tan, Zhaoying Wang, Lianju Shen, Chunlan Long, Guanghui Wei, Dawei He

Open access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. HEPPAR1 and PIWIL2 as Panel Markers for Hepatocellular Carcinoma.Asian Pacific journal of cancer prevention : APJCP · 2024
    Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Liming JinDepartment of Urology, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.
Zhaoxia ZhangDepartment of Urology, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.
Zhang WangChongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing, 400014, China.
Xiaojun TanChongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing, 400014, China.
Zhaoying WangChongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing, 400014, China.
Lianju ShenChongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing, 400014, China.
Chunlan LongChongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing, 400014, China.
Guanghui WeiDepartment of Urology, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.
Dawei HeDepartment of Urology, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. hedawei@hospital.cqmu.edu.cn.ORCID http://orcid.org/0000-0002-1695-5886
China International Science and Technology Cooperation · CN

Funding

the Special Project of Science and Technology Innovation for Social Undertakings and Livelihood Guarantee of Chongqing cstc2017shmsA130103the Special Project of Science and Technology Innovation for Social Undertakings and Livelihood Guarantee of Chongqing cstc2019jscx-tjsbX0003
6 · The paper itself

Abstract

backgroundCSCs play an important role in tumor development. Some studies have demonstrated that piRNAs participate in the progression of various cancers. However, the detailed function of piRNAs in CSCs requires further investigation. This study aimed to investigate the significance of novel piRNA MW557525, one of the top five up-regulated piRNAs screened by gene chip and it has been verified by RT-q-PCR that it is indeed the most obvious up-regulated expression in Piwil2-iCSCs. METHODS AND

resultsDifferentially expressed piRNAs in Piwil2-iCSCs were screened by gene chip. Target genes were predicted by the miRanda algorithm and subjected to GO and KEGG analysis. One of the differential piRNAs, novel piRNA MW557525, was transfected and its target gene NOP56 was silenced in Piwil2-iCSCs, respectively. RT-qPCR, western blot (WB) and dual luciferase reporter assay were used to investigate the interaction of piRNA MW557525 and NOP56. We identified the effect of piRNA MW557525 and NOP56 knockdown on cell proliferation, migration, invasion, and apoptosis via CCK-8, transwell assay, and flow cytometry. The expressions of CD24, CD133, KLF4, and SOX2 were detected via WB. The results showed that piRNA MW557525 was negatively correlated with NOP56, and it promoted the proliferation, migration, invasion, and inhibited apoptosis in Piwil2-iCSCs, and it also promoted the expressions of CD24, CD133, KLF4, and SOX2, while NOP56 showed the opposite effect.

conclusionsThese findings suggested that novel piRNA MW557525 might be a novel therapeutic target in Piwil2-iCSCs.

Indexed as

Stem CellsCell ProliferationOligonucleotide Array Sequence AnalysisRNA, Small InterferingRNA, Small InterferingApoptosisInvasionMigrationNOP56piRNAPiwil2-iCSCsProliferation

Identifiers

PMID35411481
OpenAlexW4223550815

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.