ReviewNature protocols2022
Rapid in situ identification of biological specimens via DNA amplicon sequencing using miniaturized laboratory equipment.
Review in Nature protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Omics-Guided Construction of Microbial Consortia for Reproducible Traditional Fermented Foods and Beverages.Foods (Basel, Switzerland) · 2026Review
- Cave microbial communities are structured by environmental matrix and depth and can be characterized with field-portable assays.Applied and environmental microbiology · 2026Article
- Using miniaturized laboratory equipment and DNA barcoding to improve conservation genetics training and identify illegally traded species.Conservation biology : the journal of the Society for Conservation Biology · 2026Article
- A Field-Deployable eDNA Metabarcoding Workflow Including De Novo Reference Assembly for Characterising Understudied Biodiversity Hotspots.Molecular ecology resources · 2026Article
- Innovative Systems Biology in Baijiu Fermentation: Unveiling Omics Landscapes and Microbial Synergy.Foods (Basel, Switzerland) · 2026Review
- High-throughput detection of microeukaryotic parasites in insects using Nanopore sequencing.Microbial genomics · 2026Article
- Going Mobile: Using Portable Genomic Technologies for PCR-Free In Situ Species Identification and Real-Time Molecular Systematics.Ecology and evolution · 2025Article
- Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa.Scientific data · 2025Article
- Perfoliate Pondweed Meadows in Northern Coastal Areas-Reservoirs of Diverse Bacteria With Pathogenic Traits and Complex Carbon Metabolism.Environmental microbiology reports · 2025Article
- A composite universal DNA signature for the tree of life.Nature ecology & evolution · 2025Article
- De novo clustering of large long-read transcriptome datasets with isONclust3.Bioinformatics (Oxford, England) · 2025Article
- Article
- Rapid on-site universal vertebrate species identification via multi-barcode nanopore sequencing.PloS one · 2025Article
- A new species ofIMA fungus · 2025Article
- Sequencing the orthologs of human autosomal forensic short tandem repeats provides individual- and species-level identification in African great apes.BMC ecology and evolution · 2024Article
- Microeukaryote community coalescence strengthens community stability and elevates diversity.FEMS microbiology ecology · 2024Article
- Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems.Science China. Life sciences · 2024Review
- The Applications of Nanopore Sequencing Technology in Animal and Human Virus Research.Viruses · 2024Review
- Highly Multiplexed Reverse-Transcription Loop-Mediated Isothermal Amplification and Nanopore Sequencing (LAMPore) for Wastewater-Based Surveillance.ACS ES&T water · 2024Article
- Identification of non-model mammal species using the MinION DNA sequencer from Oxford Nanopore.PeerJ · 2024Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many parts of the world, human-mediated environmental change is depleting biodiversity faster than it can be characterized, while invasive species cause agricultural damage, threaten human health and disrupt native habitats. Consequently, the application of effective approaches for rapid surveillance and identification of biological specimens is increasingly important to inform conservation and biosurveillance efforts. Taxonomic assignments have been greatly advanced using sequence-based applications, such as DNA barcoding, a diagnostic technique that utilizes PCR and DNA sequence analysis of standardized genetic regions. However, in many biodiversity hotspots, endeavors are often hindered by a lack of laboratory infrastructure, funding for biodiversity research and restrictions on the transport of biological samples. A promising development is the advent of low-cost, miniaturized scientific equipment. Such tools can be assembled into functional laboratories to carry out genetic analyses in situ, at local institutions, field stations or classrooms. Here, we outline the steps required to perform amplicon sequencing applications, from DNA isolation to nanopore sequencing and downstream data analysis, all of which can be conducted outside of a conventional laboratory environment using miniaturized scientific equipment, without reliance on Internet connectivity. Depending on sample type, the protocol (from DNA extraction to full bioinformatic analyses) can be completed within 10 h, and with appropriate quality controls can be used for diagnostic identification of samples independent of core genomic facilities that are required for alternative methods.
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