Evidence map›Paper›PMID 35410497›Full record

ArticleCephalalgia : an international journal of headache2022

Calcitonin receptor antibody validation and expression in the rodent brain.

Erica R Hendrikse, Tayla A Rees, Zoe Tasma, Christelle Le Foll, Thomas A Lutz, Andrew Siow, Peter J Wookey, Christopher S Walker, Debbie L Hay

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cephalalgia : an international journal of headache, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
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  5. Calcitonin receptor signaling in nucleus accumbens D1R- and D2R-expressing medium spiny neurons bidirectionally alters opioid taking in male rats.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023
    Article
  6. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Erica R HendrikseSchool of Biological Sciences, The University of Auckland, Auckland, New Zealand.
Tayla A ReesSchool of Biological Sciences, The University of Auckland, Auckland, New Zealand.
Zoe TasmaSchool of Biological Sciences, The University of Auckland, Auckland, New Zealand.ORCID 0000-0002-7652-6004
Christelle Le FollInstitute of Veterinary Physiology, University of Zurich, Zurich, Switzerland.
Thomas A LutzInstitute of Veterinary Physiology, University of Zurich, Zurich, Switzerland.
Andrew SiowSchool of Biological Sciences, The University of Auckland, Auckland, New Zealand.ORCID 0000-0002-0341-065X
Peter J WookeyDepartment of Medicine-Austin, The University of Melbourne, Heidelberg, Australia.
Christopher S WalkerSchool of Biological Sciences, The University of Auckland, Auckland, New Zealand.ORCID 0000-0001-8151-4123
Debbie L HayMaurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Auckland, New Zealand.ORCID 0000-0002-9558-5122
University of Auckland · NZUniversity of Zurich · CHThe University of Melbourne · AU

Funding

Target validation of a novel CGRP receptor in migraineRF1NS113839 · NINDS · UNIVERSITY OF IOWA · PI HAY, DEBBIE L, RUSSO, ANDREW F · 2019 to 2019
$3.1M
NINDS NIH HHS RF1 NS113839
6 · The paper itself

Abstract

BACKGROUND AND

aimTherapeutics that reduce calcitonin gene-related peptide activity are effective migraine treatments. However, gaps remain in our understanding of the molecular mechanisms that link calcitonin gene-related peptide to migraine. The amylin 1 receptor responds potently to calcitonin gene-related peptide, and to the related peptide amylin, but its role in relation to either peptide or to migraine is unclear. We sought to better understand the expression of the amylin 1 receptor protein subunit, the calcitonin receptor, in the rodent brain.

methodsWe profiled three antibodies for immunodetection of calcitonin receptor, using immunocytochemistry, western blotting, and calcitonin receptor conditional knockout mouse tissue. Selected migraine-relevant rat brain regions were then examined for calcitonin receptor-like immunoreactivity.

resultsAll three antibodies detected calcitonin receptor protein but only one (188/10) produced robust immunostaining in rodent brain, under the conditions used. Calcitonin receptor-like immunoreactivity was apparent in the rat brainstem and midbrain including the locus coeruleus, periaqueductal grey and spinal trigeminal nucleus.

conclusionsAnti-calcitonin receptor antibodies require comprehensive profiling to ensure confidence in the detection of calcitonin receptor. Using a validated antibody, calcitonin receptor-like immunoreactivity was detected in several brain regions relevant to migraine. Further research is needed to understand the functional consequences of calcitonin receptor expression for calcitonin gene-related peptide or amylin physiology and pathophysiology.

Indexed as

Calcitonin Gene-Related PeptideMigraine DisordersAnimalsBrainIslet Amyloid PolypeptideMiceRatsReceptors, CalcitoninReceptors, Calcitonin Gene-Related PeptideReceptors, Islet Amyloid PolypeptideCalcitonin Gene-Related PeptideIslet Amyloid PolypeptideReceptors, CalcitoninReceptors, Calcitonin Gene-Related PeptideReceptors, Islet Amyloid PolypeptideAMY1amylinCGRPG protein-coupled receptormigrainepain

Identifiers

PMID35410497
PMCPMC9441190
OpenAlexW4223491630

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.