ArticleJournal of animal science and biotechnology2022
miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1.
Article in Journal of animal science and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- The crosslink between autophagy and ferroptosis in polycystic ovary syndrome: from synergistic pathogenesis to targeted therapy.Journal of ovarian research · 2026Review
- Analysis of the resistance of small peptides from Periplaneta americana to HScientific reports · 2026Article
- ceRNA regulatory networks reveals gga-miR-30b-5p as a regulator of granulosa cell apoptosis via targeting NR5A2 in atrophic ovaries of broody chickens.Poultry science · 2026Article
- Circular RNA profiling revealed an evolutionarily conserved circACACA promotes liver lipid metabolism, oxidative stress, and autophagy disorder in a ceRNA manner.PLoS genetics · 2025Article
- Mechanisms of programmed cell death in livestock follicular development and atresia: a review.Journal of animal science and biotechnology · 2025Review
- Integrated analysis of microbiome and transcriptome reveals the mechanisms underlying the chlorogenic acid-mediated attenuation of oxidative stress and systemic inflammatory responses via gut-liver axis in post-peaking laying hens.Journal of animal science and biotechnology · 2025Article
- Autophagy Inhibition and Sensitization to Cisplatin in Esophageal Cancer Stem-like Cells via All-trans Retinoic Acid-induced miR-30a.Current medicinal chemistry · 2025Article
- Whole-transcriptome insights into follicle selection: deciphering key regulatory networks in Luxi gamecock.Frontiers in genetics · 2025Article
- Growth differentiation factor 9 activates the TGF-β pathway in follicle atresia of Muscovy ducks.Poultry science · 2024Article
- Selenized Yeast Protects Against Cadmium-Induced Follicular Atresia in Laying Hens by Reducing Autophagy in Granulosa Cells.Current issues in molecular biology · 2024Article
- The Beneficial Effects ofInternational journal of molecular sciences · 2024Article
- High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein.Journal of animal science and biotechnology · 2024Article
- miRNA profiling of chicken follicles during follicular development.Scientific reports · 2024Article
- MiR-134-3p targets HMOX1 to inhibit ferroptosis in granulosa cells of sheep follicles.Journal of ovarian research · 2024Article
- Expression profiles of circulating miRNAs in an endangered Piedmontese sheep breed during the estrus cycle.Frontiers in veterinary science · 2024Article
- Whole transcriptome analysis revealed the regulatory network and related pathways of non-coding RNA regulating ovarian atrophy in broody hens.Frontiers in veterinary science · 2024Article
- The constructive and destructive impact of autophagy on both genders' reproducibility, a comprehensive review.Autophagy · 2023Review
- Mechanisms of and Potential Medications for Oxidative Stress in Ovarian Granulosa Cells: A Review.International journal of molecular sciences · 2023Review
- Untargeted Metabolomics Revealed Potential Biomarkers of Small Yellow Follicles of Chickens during Sexual Maturation.Metabolites · 2023Article
- Interacting Networks of the Hypothalamic-Pituitary-Ovarian Axis Regulate Layer Hens Performance.Genes · 2023Review
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Authors and funding
17 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundThe egg production performance of chickens is affected by many factors, including genetics, nutrition and environmental conditions. These factors all play a role in egg production by affecting the development of follicles. MicroRNAs (miRNAs) are important non-coding RNAs that regulate biological processes by targeting genes or other non-coding RNAs after transcription. In the animal reproduction process, miRNA is known to affect the development and atresia of follicles by regulating apoptosis and autophagy of granulosa cells (GCs).
resultsIn this study, we identified potential miRNAs in the atretic follicles of broody chickens and unatretic follicles of healthy chickens. We identified gga-miR-30a-5p in 50 differentially expressed miRNAs and found that gga-miR-30a-5p played a regulatory role in the development of chicken follicles. The function of miR-30a-5p was explored through the transfection test of miR-30a-5p inhibitor and miR-30a-5p mimics. In the study, we used qPCR, western blot and flow cytometry to detect granulosa cell apoptosis, autophagy and steroid hormone synthesis. Confocal microscopy and transmission electron microscopy are used for the observation of autophagolysosomes. The levels of estradiol (E2), progesterone (P4), malondialdehyde (MDA) and superoxide dismutase (SOD) were detected by ELISA. The results showed that miR-30a-5p showed a negative effect on autophagy and apoptosis of granulosa cells, and also contributed in steroid hormones and reactive oxygen species (ROS) production. In addition, the results obtained from the biosynthesis and dual luciferase experiments showed that Beclin1 was the target gene of miR-30a-5p. The rescue experiment conducted further confirmed that Beclin1 belongs to the miR-30a-5p regulatory pathway.
conclusionsIn summary, after deep miRNA sequencing on healthy and atretic follicles, the results indicated that miR-30a-5p inhibits granulosa cell death by inhibiting Beclin1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.