ArticleCommunications biology2022
Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- The combination of morphogenic regulators BABY BOOM and GRF-GIF improves maize transformation efficiency and promotes leaf regeneration.The New phytologist · 2026Article
- Boosting genome editing in perennial plants by CRISPR-Combo mediated morphogenic gene activation.Nature communications · 2026Article
- AI-Guided DNA-Free and Genotype-Independent Genome Editing for Soybean Improvement.Plants (Basel, Switzerland) · 2026Review
- Improving iSpyMacCas9 multiplex genome editing in rice by CRISPR-combo-mediated BBM1 activation.The Plant journal : for cell and molecular biology · 2026Article
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- FUL1 is a key regulator of grain morphology, stem development, and other shoot characteristics inaBIOTECH · 2026Article
- WUSCHEL Transcription Factor: From Stem Cell Maintenance to Crop Improvement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Induction of Synthetic Apomixis in Two Sorghum Hybrids Enables Seed Yield and Genotype Preservation Over Multiple Generations.Plant biotechnology journal · 2026Article
- Molecular Regulators of In Vitro Regeneration in Wheat: Roles of Morphogenic Factors in Transformation, Genome Editing, and Breeding.International journal of molecular sciences · 2026Review
- Optimized Protocol for Sorghum Regeneration: Enhancing Embryogenic Callus Formation from Immature Inflorescences.Recent patents on biotechnology · 2026Article
- Biotechnological and genetic innovations to enhance sorghum adaptation under climate change.Frontiers in plant science · 2026Review
- Establishment of an efficientFrontiers in plant science · 2026Article
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Efficient and cost-effective shoot regeneration in Aguaymanto (Physalis peruviana L.) using meta-Topolin and 6-benzylaminopurine combinations.Journal, genetic engineering & biotechnology · 2025Article
- Mechanism and Application of Developmental Factors in Plant Genetic Transformation.International journal of molecular sciences · 2025Review
- Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.).Plant biotechnology journal · 2025Article
- Morphogenetic Factors as a Tool for Enhancing Plant Regeneration Capacity During In Vitro Transformation.International journal of molecular sciences · 2025Review
- Identification of quantitative trait loci for in vitro plant regeneration from leaf microexplants in cucumber (Cucumis sativus L.).Journal of applied genetics · 2025Article
- Emerging trends in transgene-free crop development: insights into genome editing and its regulatory overview.Plant molecular biology · 2025Review
- Efficient and versatile rapeseed transformation for new breeding technologies.The Plant journal : for cell and molecular biology · 2025Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For many important crops including sorghum, use of CRISPR/Cas technology is limited not only by the delivery of the gene-modification components into a plant cell, but also by the ability to regenerate a fertile plant from the engineered cell through tissue culture. Here, we report that Wuschel2 (Wus2)-enabled transformation increases not only the transformation efficiency, but also the CRISPR/Cas-targeted genome editing frequency in sorghum (Sorghum bicolor L.). Using Agrobacterium-mediated transformation, we have demonstrated Wus2-induced direct somatic embryo formation and regeneration, bypassing genotype-dependent callus formation and significantly shortening the tissue culture cycle time. This method also increased the regeneration capacity that resulted in higher transformation efficiency across different sorghum varieties. Subsequently, advanced excision systems and "altruistic" transformation technology have been developed to generate high-quality morphogenic gene-free and/or selectable marker-free sorghum events. Finally, we demonstrate up to 6.8-fold increase in CRISPR/Cas9-mediated gene dropout frequency using Wus2-enabled transformation, compared to without Wus2, across various targeted loci in different sorghum genotypes.
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