Evidence map›Paper›PMID 35408969›Full record

ArticleInternational journal of molecular sciences2022

MicroRNAs Expression in Response to rhNGF in Epithelial Corneal Cells: Focus on Neurotrophin Signaling Pathway.

Chiara Compagnoni, Veronica Zelli, Andrea Bianchi, Antinisca Di Marco, Roberta Capelli, Davide Vecchiotti, Laura Brandolini, Anna Maria Cimini, Francesca Zazzeroni, Marcello Allegretti and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Chiara CompagnoniDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.
Veronica ZelliDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.
Andrea BianchiDepartment of Information Engineering, Computer Science and Mathematics, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.ORCID 0000-0002-6046-4355
Antinisca Di MarcoDepartment of Information Engineering, Computer Science and Mathematics, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.ORCID 0000-0001-7214-9945
Roberta CapelliDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.
Davide VecchiottiDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.ORCID 0000-0003-1054-4565
Laura BrandoliniDompé Farmaceutici Spa, via Campo di Pile, 1, 67100 L'Aquila, Italy.ORCID 0000-0002-5959-4443
Anna Maria CiminiDepartment of Life, Health and Environmental Sciences, University of L'Aquila, P.zza S. Tommasi, 67100 L'Aquila, Italy.ORCID 0000-0002-2737-7970
Francesca ZazzeroniDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.
Marcello AllegrettiDompé Farmaceutici Spa, via Campo di Pile, 1, 67100 L'Aquila, Italy.ORCID 0000-0002-0617-0233
Edoardo AlesseDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.
Alessandra TessitoreDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.
University of L'Aquila · ITDompé (Italy) · IT

Funding

Decree of Italian Ministry for Economical Progress 2018 Project n°230 "Identification of new compounds for treatment of eye and ear diseases" to EADompé (Italy) 07_CONTRACT_DOMPE_TESSITORE for the project entitled 'MicroRNA expression analysis in response to different NGF bioformulations in in vitro corneal cell models' to AT
6 · The paper itself

Abstract

purposeNerve growth factor efficacy was demonstrated for corneal lesions treatment, and recombinant human NGF (rhNGF) was approved for neurotrophic keratitis therapy. However, NGF-induced molecular responses in cornea are still largely unknown. We analyzed microRNAs expression in human epithelial corneal cells after time-dependent rhNGF treatment.

methodsNearly 700 microRNAs were analyzed by qRT-PCR. MicroRNAs showing significant expression differences were examined by DIANA-miRpath v.3.0 to identify target genes and pathways. Immunoblots were performed to preliminarily assess the strength of the in silico results.

resultsTwenty-one microRNAs (miR-26a-1-3p, miR-30d-3p, miR-27b-5p, miR-146a-5p, miR-362-5p, mir-550a-5p, mir-34a-3p, mir-1227-3p, mir-27a-5p, mir-222-5p, mir-151a-5p, miR-449a, let7c-5p, miR-337-5p, mir-29b-3p, miR-200b-3p, miR-141-3p, miR-671-3p, miR-324-5p, mir-411-3p, and mir-425-3p) were significantly regulated in response to rhNGF. In silico analysis evidenced interesting target genes and pathways, including that of neurotrophin, when analyzed in depth. Almost 80 unique target genes (e.g.,

conclusionsMicroRNA levels in response to rhNGF were for the first time analyzed in corneal cells. Novel insights about microRNAs, target genes, pathways modulation, and possible biological responses were provided. Importantly, given the putative role of microRNAs as biomarkers or therapeutic targets, our results make available data which might be potentially exploitable for clinical applications.

Indexed as

MicroRNAsNerve Growth FactorCorneaHumansProto-Oncogene Proteins c-aktSignal TransductionMicroRNAsMIRN324 microRNA, humanMirn337 microRNA, humanMIRN411 microRNA, humanNerve Growth FactorProto-Oncogene Proteins c-aktbiomarkerscorneal diseasesmicroRNAneurotrophin signaling pathwayrecombinant human NGF (rhNGF)

Identifiers

PMID35408969
PMCPMC8998691
OpenAlexW4220877257

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.