ArticleInternational journal of molecular sciences2022
An Efficient and Universal Protoplast Isolation Protocol Suitable for Transient Gene Expression Analysis and Single-Cell RNA Sequencing.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants.Plants (Basel, Switzerland) · 2026Review
- An efficient method for tissue-specific protoplast isolation suitable for single-cell RNA sequencing and transient gene expression analysis in saffron (Crocus sativus L.).BMC biotechnology · 2026Article
- Epigenetic regulation of fruit shape determination by the JAGGED gene in Capsella rubella.Nature communications · 2026Article
- Review
- Assembly of Arundinella anomala genome to facilitate single-cell resolved functional and developmental characterization of CNature plants · 2026Article
- Why "Where" Matters as Much as "How Much": Single-Cell and Spatial Transcriptomics in Plants.International journal of molecular sciences · 2025Review
- Single-cell omics in plant biology: mechanistic insights and applications for crop improvement.Advanced biotechnology · 2025Review
- Evolution of a SHOOTMERISTEMLESS transcription factor binding site promotes fruit shape determination.Nature plants · 2025Article
- Application of an Efficient Enhancer in Gene Function Research.Plants (Basel, Switzerland) · 2024Article
- A Comparison of DNA-Methylation during Protoplast Culture of Ponkan Mandarin (Plants (Basel, Switzerland) · 2024Article
- Quantitative and spatially resolved detection of multiplexed microRNA from plant tissue via hybridization to hydrogel-bound DNA probes in nanoliter well arrays.Microsystems & nanoengineering · 2024Article
- Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling.Plant methods · 2024Article
- A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza.Plant cell reports · 2024Article
- Genetic transformation in conifers: current status and future prospects.Forestry research · 2024Review
- Recent advances in exploring transcriptional regulatory landscape of crops.Frontiers in plant science · 2024Review
- Advances in the Application of Single-Cell Transcriptomics in Plant Systems and Synthetic Biology.Biodesign research · 2024Review
- Isolation, Purification, and Application of Protoplasts and Transient Expression Systems in Plants.International journal of molecular sciences · 2023Review
- Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023Article
- Application of a novel strong promoter from Chinese fir (Frontiers in plant science · 2023Article
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Authors and funding
7 authors.
Funding
Abstract
The recent advent of single-cell RNA sequencing (scRNA-seq) has enabled access to the developmental landscape of a complex organ by monitoring the differentiation trajectory of every specialized cell type at the single-cell level. A main challenge in this endeavor is dissociating plant cells from the rigid cell walls and some species are recalcitrant to such cellular isolation. Here, we describe the establishment of a simple and efficient protocol for protoplast preparation in
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