Evidence map›Paper›PMID 35406547›Full record

ArticleCancers2022

Minimally Invasive Preclinical Monitoring of the Peritoneal Cavity Tumor Microenvironment.

Zachary Spencer Dunn, Yan-Ruide Li, Yanqi Yu, Derek Lee, Alicia Gibbons, James Joon Kim, Tian Yang Zhou, Mulin Li, Mya Nguyen, Xinjian Cen and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 3 citations in OpenAlex.

  1. Pooled it
  2. AdultBiomolecules · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Zachary Spencer DunnMork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA.ORCID 0000-0003-2500-6006
Yan-Ruide LiDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Yanqi YuDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0002-6969-3752
Derek LeeDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Alicia GibbonsDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0003-0567-375X
James Joon KimDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0001-7597-4991
Tian Yang ZhouDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Mulin LiDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0002-1296-8268
Mya NguyenDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0003-4975-6231
Xinjian CenDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Yang ZhouDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Pin WangMork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA.
Lili YangDepartment of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
University of California, Los Angeles · USUniversity of Southern California · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intraperitoneal (i.p.) experimental models in mice can recapitulate the process of i.p. dissemination in abdominal cancers and may help uncover critical information about future successful clinical treatments. i.p. cellular composition is studied in preclinical models addressing a wide spectrum of other pathophysiological states such as liver cirrhosis, infectious disease, autoimmunity, and aging. The peritoneal cavity is a multifaceted microenvironment that contains various immune cell populations, including T, B, NK, and various myeloid cells, such as macrophages. Analysis of the peritoneal cavity is often obtained by euthanizing mice and performing terminal peritoneal lavage. This procedure inhibits continuous monitoring of the peritoneal cavity in a single mouse and necessitates the usage of more mice to assess the cavity at multiple timepoints, increasing the cost, time, and variability of i.p. studies. Here, we present a simple, novel method termed in vivo intraperitoneal lavage (IVIPL) for the minimally invasive monitoring of cells in the peritoneal cavity of mice. In this proof-of-concept, IVIPL provided real-time insights into the i.p. tumor microenvironment for the development and study of ovarian cancer therapies. Specifically, we studied CAR-T cell therapy in a human high-grade serous ovarian cancer (HGSOC) xenograft mouse model, and we studied the immune composition of the i.p. tumor microenvironment (TME) in a mouse HGSOC syngeneic model.

Indexed as

cell therapyimmune cell monitoringintraperitoneal modelspreclinical ovarian cancer

Identifiers

PMID35406547
PMCPMC8997523
OpenAlexW4220919611

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.