ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022
Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- Granular Hydrogel Composites as Matrices for Engineered Living Materials.ACS applied polymer materials · 2026Article
- Soft, Reactive, and Alive: A Dynamic Framework for Degradation and Functional Stability of Polymeric Biomaterials.Polymers · 2026Review
- Modeling cancer with bacteria-integrated tumor microenvironments using biomaterials: Emerging concepts and opportunities.Materials today. Bio · 2026Review
- Self-Growing and Mechanically Tunable Living Materials withACS applied polymer materials · 2026Article
- PEG-Based Living Hydrogels Engineered for Tunable Production of Bioactive Lipopeptides.ACS applied polymer materials · 2026Article
- Selective Covalent Assembly of Gram-Negative Bacteria and Synthetic Polymers into Functional Living Materials.Journal of the American Chemical Society · 2026Article
- High-throughput bioprinted 3D cultures for probing host-pathogen interactions in bioinspired microenvironments.RSC applied polymers · 2026Article
- Hydrogel platforms for engineered live biotherapeutics: materials, microbial integration and clinical potential.RSC pharmaceutics · 2026Review
- Evaluating Cytocompatibility of Corynebacterium glutamicum-poly Vinyl Alcohol Living Biomaterials for Ocular Use.Advanced healthcare materials · 2026Article
- PEARL: Protein Eluting Alginate with Recombinant Lactobacilli.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Review
- A multicellular self-organized probiotic platform for oral delivery enhances intestinal colonization.Nature communications · 2025Article
- Controlled Release of Microorganisms from Engineered Living Materials.ACS applied materials & interfaces · 2025Article
- Mechanics control the proliferation of diatoms entrapped in hydrogels.Soft matter · 2025Article
- Adaptations of Gram-Negative and Gram-Positive Probiotic Bacteria in Engineered Living Materials.ACS biomaterials science & engineering · 2025Article
- A multifunctional living hydrogel for the synergistic management of infected diabetic wounds.Materials today. Bio · 2025Article
- Preclinical Assessment of Living Therapeutic Materials: State-of-Art and Challenges.ACS biomaterials science & engineering · 2025Review
- Growth of nonmotile stress-responsive bacteria in 3D colonies under confining pressure.Biophysical journal · 2025Article
- A Comparative Study between Thiol-Ene and Acrylate Photocrosslinkable Hyaluronic Acid Hydrogel Inks for Digital Light Processing.Macromolecular bioscience · 2025Article
- Hyper-porous encapsulation of microbes for whole cell biocatalysis and biomanufacturing.Microbial cell factories · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Engineered living materials (ELMs) are a new class of materials in which living organism incorporated into diffusive matrices uptake a fundamental role in material's composition and function. Understanding how the spatial confinement in 3D can regulate the behavior of the embedded cells is crucial to design and predict ELM's function, minimize their environmental impact and facilitate their translation into applied materials. This study investigates the growth and metabolic activity of bacteria within an associative hydrogel network (Pluronic-based) with mechanical properties that can be tuned by introducing a variable degree of acrylate crosslinks. Individual bacteria distributed in the hydrogel matrix at low density form functional colonies whose size is controlled by the extent of permanent crosslinks. With increasing stiffness and elastic response to deformation of the matrix, a decrease in colony volumes and an increase in their sphericity are observed. Protein production follows a different pattern with higher production yields occurring in networks with intermediate permanent crosslinking degrees. These results demonstrate that matrix design can be used to control and regulate the composition and function of ELMs containing microorganisms. Interestingly, design parameters for matrices to regulate bacteria behavior show similarities to those elucidated for 3D culture of mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.